DNA detection microfluidic device based on negative dielectrophoresis of DNA labeled microbeads

Zhenhao Ding, Kenichi Ida, Kenya Matsuda, Michihiko Nakano, Junya Suehiro

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In this study, we focus on the alteration of negative dielectrophoresis (n-DEP) caused by small amount of DNA labeling on the microbeads and propose a new design of microfluidic device for more sensitive DNA detection. In previous study, we proposed a new electrical technique for rapid detection of DNA amplified by polymerase chain reaction (PCR). The method is based on dramatic alteration of dielectrophoresis (DEP) characteristics of microbeads caused by DNA labeling. The DNA labeling of microbeads alters their DEP behavior in such a way that they are trapped on a microelectrode under the action of positive DEP (p-DEP), whereas bare microbeads are not, due to the n-DEP. However, this method requires at least 105 copies of DNA labelling on a microbead to alter the DEP behavior of microbeads from negative to positive. As a result, we are able to distinguish bare microbeads and microbeads labeled with DNA at DNA to microbead ratio of 3×103: 1 and 3×104: 1, based on the n-DEP alteration.

Original languageEnglish
Title of host publicationIEEE SENSORS 2017 - Conference Proceedings
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages1-3
Number of pages3
Volume2017-December
ISBN (Electronic)9781509010127
DOIs
Publication statusPublished - Dec 21 2017
Event16th IEEE SENSORS Conference, ICSENS 2017 - Glasgow, United Kingdom
Duration: Oct 30 2017Nov 1 2017

Other

Other16th IEEE SENSORS Conference, ICSENS 2017
CountryUnited Kingdom
CityGlasgow
Period10/30/1711/1/17

All Science Journal Classification (ASJC) codes

  • Electrical and Electronic Engineering

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  • Cite this

    Ding, Z., Ida, K., Matsuda, K., Nakano, M., & Suehiro, J. (2017). DNA detection microfluidic device based on negative dielectrophoresis of DNA labeled microbeads. In IEEE SENSORS 2017 - Conference Proceedings (Vol. 2017-December, pp. 1-3). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/ICSENS.2017.8234345