DNA Detection using Microbeads-based Dielectrophoretic Impedance Measurement

Michihiko Nakano, Zhenhao Ding, Hiromichi Kasahara, Junya Suehiro

Research output: Contribution to journalConference article

3 Citations (Scopus)

Abstract

A new electrical technique for rapid detection of DNA amplified by polymerase chain reaction (PCR) using dielectrophoresis (DEP) of microbeads was demonstrated. The amplicons were chemically immobilized on the microbeads. The DNA immobilization on the microbeads alters their DEP behavior in such a way that they are trapped on a microelectrode under the action of positive DEP, whereas pristine microbeads are not. Combining the dramatic alteration in DEP characteristics with impedance measurement leads to rapid and quantitative detection of the amplicon in a few seconds. The proposed microbead-based assay could be applicable for rapid, quantitative, and automated detection of virus infection.

Original languageEnglish
Article number6985174
Pages (from-to)1010-1013
Number of pages4
JournalProceedings of IEEE Sensors
Volume2014-December
Issue numberDecember
DOIs
Publication statusPublished - Dec 12 2014
Event13th IEEE SENSORS Conference, SENSORS 2014 - Valencia, Spain
Duration: Nov 2 2014Nov 5 2014

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Electrophoresis
DNA
immobilization
polymerase chain reaction
Microelectrodes
Polymerase chain reaction
virus
Viruses
assay
Assays
detection

All Science Journal Classification (ASJC) codes

  • Electrical and Electronic Engineering

Cite this

DNA Detection using Microbeads-based Dielectrophoretic Impedance Measurement. / Nakano, Michihiko; Ding, Zhenhao; Kasahara, Hiromichi; Suehiro, Junya.

In: Proceedings of IEEE Sensors, Vol. 2014-December, No. December, 6985174, 12.12.2014, p. 1010-1013.

Research output: Contribution to journalConference article

Nakano, Michihiko ; Ding, Zhenhao ; Kasahara, Hiromichi ; Suehiro, Junya. / DNA Detection using Microbeads-based Dielectrophoretic Impedance Measurement. In: Proceedings of IEEE Sensors. 2014 ; Vol. 2014-December, No. December. pp. 1010-1013.
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