DNA hybridization in nanostructural molecular assemblies enables detection of gene mutations without a fluorescent probe

Tatsuo Maruyama, Lian Chun Park, Toshimitsu Shinohara, Masahiro Goto

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

We have developed a simple single nucleotide polymorphisms (SNPs) analysis utilizing DNA hybridization in nanostructural molecular assemblies. The novel technique enables the detection of a single-base mismatch in a DNA sequence without a fluorescent probe. This report describes for the first time that DNA hybridization occurs in the nanostructural molecular assemblies (termed reverse micelles) formed in an organic medium. The restricted nanospace in the reverse micelles amplifies the differences in the hybridization rate between mismatched and perfectly matched DNA probes. For a model system, we hybridized a 20-mer based on the p53 gene sequence to 20-mer complementary oligonucleotides with various types of mismatches. Without any DNA labeling or electrochemical apparatus, we successfully detected the various oligonucleotide mismatches by simply measuring the UV absorbance at 260 nm.

Original languageEnglish
Pages (from-to)49-53
Number of pages5
JournalBiomacromolecules
Volume5
Issue number1
DOIs
Publication statusPublished - Jan 1 2004

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biomaterials
  • Polymers and Plastics
  • Materials Chemistry

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