DNA hybridization in reverse micelles and its application to mutation detection

Lian Chun Park, Tatsuo Maruyama, Masahiro Goto

    Research output: Contribution to journalArticlepeer-review

    18 Citations (Scopus)

    Abstract

    DNA hybridization was investigated in AOT (sodium di-2-ethylhexyl sulfosuccinate)/isooctane reverse micelles. The single-stranded DNA molecules were encapsulated in the nanoscale water pools formed in the reverse micelles, reducing the hybridization rate. The DNA hybridization can be monitored by simply measuring the UV absorbance of the reverse micellar solution at 260 nm. We found that the DNA hybridization occurred only at the restricted water content (Wo = [H2O]/[AOT] = 20) and below room temperature. We applied this DNA hybridization behavior in reverse micelles to mutation detection in a model gene p53 and successfully detected the single nucleotide mutations in 20-mer, 30-mer and 50-mer nucleotides without a DNA labeling.

    Original languageEnglish
    Pages (from-to)161-165
    Number of pages5
    JournalAnalyst
    Volume128
    Issue number2
    DOIs
    Publication statusPublished - Feb 1 2003

    All Science Journal Classification (ASJC) codes

    • Analytical Chemistry
    • Biochemistry
    • Environmental Chemistry
    • Spectroscopy
    • Electrochemistry

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