DNA hybridization in reverse micelles and its application to mutation detection

Lian Chun Park, Tatsuo Maruyama, Masahiro Goto

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

DNA hybridization was investigated in AOT (sodium di-2-ethylhexyl sulfosuccinate)/isooctane reverse micelles. The single-stranded DNA molecules were encapsulated in the nanoscale water pools formed in the reverse micelles, reducing the hybridization rate. The DNA hybridization can be monitored by simply measuring the UV absorbance of the reverse micellar solution at 260 nm. We found that the DNA hybridization occurred only at the restricted water content (Wo = [H2O]/[AOT] = 20) and below room temperature. We applied this DNA hybridization behavior in reverse micelles to mutation detection in a model gene p53 and successfully detected the single nucleotide mutations in 20-mer, 30-mer and 50-mer nucleotides without a DNA labeling.

Original languageEnglish
Pages (from-to)161-165
Number of pages5
JournalAnalyst
Volume128
Issue number2
DOIs
Publication statusPublished - Feb 1 2003

    Fingerprint

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy
  • Electrochemistry

Cite this