DNA manipulation and retrieval from an aqueous solution with micromachined nanotweezers

Gen Hashiguchi, Takushi Goda, Maho Hosogi, Ken Hirano, Noritada Kaji, Yoshinobu Baba, Kuniyuki Kakushima, Hiroyuki Fujita

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79 Citations (Scopus)

Abstract

We have demonstrated DNA handling with micromachined nanotweezers that consist of a pair of opposing nanoprobes and integrated thermal expansion microactuators for changing the probe gap. The probe tips coated with a thin Al layer were dipped into a droplet of a solution containing λ-DNA molecules labeled with fluorescence dye, and then an ac electric field was applied between probes for several seconds. DNA molecules were then captured between the probe tips and retrieved from the solution to the air. The DNA capture between the probe tips could be performed more successfully on the droplet surface than in the underwater region. We also conducted an observation of the retrieved DNA molecules by transmission electron microscope and found that the thickness of the retrieved DNA molecules under the condition of this experiment was ∼21 nm when the time of the applied ac power (1 MHz, 20 Vpp) was 20 s.

Original languageEnglish
Pages (from-to)4347-4350
Number of pages4
JournalAnalytical chemistry
Volume75
Issue number17
DOIs
Publication statusPublished - Sep 1 2003

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

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    Hashiguchi, G., Goda, T., Hosogi, M., Hirano, K., Kaji, N., Baba, Y., Kakushima, K., & Fujita, H. (2003). DNA manipulation and retrieval from an aqueous solution with micromachined nanotweezers. Analytical chemistry, 75(17), 4347-4350. https://doi.org/10.1021/ac034501p