DNA methylation mapping in nanoslit devices at a single molecule level

Mayu Mizutani, Takao Yasui, Nortada Kaji, Sakon Rahong, Takeshi Yanagida, Masaki Kanai, Kazuki Nagashima, Tomoji Kawai, Yoshinobu Baba

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We demonstrated methylation mapping of DNA molecules specifically in nanoslit devices by using the quantum dots (QDs) combined with methyl-CpG-binding domain protein (MBDp) at a single molecule level. We fabricated nanoslit devices for fully elongation of DNA molecules. We measured the methylation sites using QDs-MBDp complexes in the nanoslit devices at a single molecule level. We found that these methylation sites showed a good agreement with the enzymatic sites of the methyltransferase.

Original languageEnglish
Title of host publication17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
PublisherChemical and Biological Microsystems Society
Pages29-31
Number of pages3
ISBN (Print)9781632666246
Publication statusPublished - Jan 1 2013
Externally publishedYes
Event17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 - Freiburg, Germany
Duration: Oct 27 2013Oct 31 2013

Publication series

Name17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
Volume1

Other

Other17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
CountryGermany
CityFreiburg
Period10/27/1310/31/13

All Science Journal Classification (ASJC) codes

  • Bioengineering

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  • Cite this

    Mizutani, M., Yasui, T., Kaji, N., Rahong, S., Yanagida, T., Kanai, M., Nagashima, K., Kawai, T., & Baba, Y. (2013). DNA methylation mapping in nanoslit devices at a single molecule level. In 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 (pp. 29-31). (17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013; Vol. 1). Chemical and Biological Microsystems Society.