DNA-Modified Electrodes

Molecular Recognition and Electrochemical Response

Koji Nakano, Shinji Uchida, Yoshiharu Mitsuhashi, Yuji Fujita, Hiroaki Taira, Mizuo Maeda

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

A biosensor for the detection of the compounds that bind to DNA is reported. A chemical derivatization of terminal phosphate ends of DNA double strands (ds) with 2-hydroxyethyl disulfide was made to immobilize DNA onto Au surfaces via chemisorption. By taking advantages of the redox couple-mediated artificial ion-channel principle, the DMA-modified electrode was successfully applied for a bioaffinity sensor. For example, cyclic voltammograms (CV) of ferrocyanide/ferricyanide couple with the DNA-modified electrode gave the redox wave due to the reversible electrode reaction. The CV peak currents were significantly enhanced on adding quinacrine. The peak currents showed almost a linear relationship with the concentration of quinacrine in the range of 10-7 - 5 × 10-7 M, and then saturated beyond the concentration of 8 × 10-7 M. CV responses toward a variety of DNA-binding substrates including quinacrine, acridine orange, safranin, spermine and spermidine showed a reasonable selectivity order according to the DNA-binding affinity of these compounds, while the response was quite small for methyl viologen which binds to ds DNAs through a nonspecific electrostatic interaction. The sensitivity was diminished when the immobilized ds DNAs were heat-denatured. The voltammetric response is primarily due to a "titration" of the immobilized ds DNAs by DNA-binding molecules. Thus the response-concentration profile for quinacrine was analyzed by using the Langmuir's isotherm. The apparent binding constant thus obtained was 1.3 × 106 M-1 which agreed fairly well with that in literatures (1.5 × 106 M-1).

Original languageEnglish
Pages (from-to)34-45
Number of pages12
JournalACS Symposium Series
Volume690
Publication statusPublished - Dec 1 1998

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Molecular recognition
DNA
Electrodes
Quinacrine
Acridine Orange
Paraquat
Spermidine
Spermine
Dynamic mechanical analysis
Chemisorption
Coulomb interactions
Titration
Ion Channels
Biosensors
Isotherms
Phosphates

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Chemical Engineering(all)

Cite this

Nakano, K., Uchida, S., Mitsuhashi, Y., Fujita, Y., Taira, H., & Maeda, M. (1998). DNA-Modified Electrodes: Molecular Recognition and Electrochemical Response. ACS Symposium Series, 690, 34-45.

DNA-Modified Electrodes : Molecular Recognition and Electrochemical Response. / Nakano, Koji; Uchida, Shinji; Mitsuhashi, Yoshiharu; Fujita, Yuji; Taira, Hiroaki; Maeda, Mizuo.

In: ACS Symposium Series, Vol. 690, 01.12.1998, p. 34-45.

Research output: Contribution to journalArticle

Nakano, K, Uchida, S, Mitsuhashi, Y, Fujita, Y, Taira, H & Maeda, M 1998, 'DNA-Modified Electrodes: Molecular Recognition and Electrochemical Response', ACS Symposium Series, vol. 690, pp. 34-45.
Nakano K, Uchida S, Mitsuhashi Y, Fujita Y, Taira H, Maeda M. DNA-Modified Electrodes: Molecular Recognition and Electrochemical Response. ACS Symposium Series. 1998 Dec 1;690:34-45.
Nakano, Koji ; Uchida, Shinji ; Mitsuhashi, Yoshiharu ; Fujita, Yuji ; Taira, Hiroaki ; Maeda, Mizuo. / DNA-Modified Electrodes : Molecular Recognition and Electrochemical Response. In: ACS Symposium Series. 1998 ; Vol. 690. pp. 34-45.
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