DNA polymerases and DNA ligases

Research output: Chapter in Book/Report/Conference proceedingChapter

4 Citations (Scopus)

Abstract

DNA polymerase and DNA ligase are ubiquitous enzymes that synthesize complementary DNA strands according to the template DNA and ligate breaks in the backbone structure of DNA, respectively, in living organisms. Multiple enzymes have been identified from each organism, and the functional sharing of these enzymes has been investigated for both DNA polymerase and DNA ligase. In addition to their fundamental role in maintaining genome integrity during replication and repair, DNA polymerases and DNA ligases are widely used for genetic engineering techniques, including DNA cloning, dideoxy sequencing, DNA labeling, mutagenesis, and other in vitro DNA manipulations. Thermostable DNA polymerases and DNA ligases are especially important in PCR and LDR/LCR, which are indispensable techniques for gene manipulation. In this chapter, we have summarized the recent developments in DNA polymerases and DNA ligases from thermophilic microbes.

Original languageEnglish
Title of host publicationThermophilic Microbes in Environmental and Industrial Biotechnology
Subtitle of host publicationBiotechnology of Thermophiles
PublisherSpringer Netherlands
Pages429-457
Number of pages29
ISBN (Electronic)9789400758995
ISBN (Print)9789400758988
DOIs
Publication statusPublished - Jan 1 2013

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DNA Ligases
DNA-Directed DNA Polymerase
DNA
Enzymes
Genetic Techniques
DNA Breaks
Genetic Engineering
DNA Sequence Analysis
Mutagenesis
Organism Cloning
Complementary DNA
Genome
Polymerase Chain Reaction
Genes

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

Ishino, S., & Ishino, Y. (2013). DNA polymerases and DNA ligases. In Thermophilic Microbes in Environmental and Industrial Biotechnology: Biotechnology of Thermophiles (pp. 429-457). Springer Netherlands. https://doi.org/10.1007/978-94-007-5899-5_17

DNA polymerases and DNA ligases. / Ishino, Sonoko; Ishino, Yoshizumi.

Thermophilic Microbes in Environmental and Industrial Biotechnology: Biotechnology of Thermophiles. Springer Netherlands, 2013. p. 429-457.

Research output: Chapter in Book/Report/Conference proceedingChapter

Ishino, S & Ishino, Y 2013, DNA polymerases and DNA ligases. in Thermophilic Microbes in Environmental and Industrial Biotechnology: Biotechnology of Thermophiles. Springer Netherlands, pp. 429-457. https://doi.org/10.1007/978-94-007-5899-5_17
Ishino S, Ishino Y. DNA polymerases and DNA ligases. In Thermophilic Microbes in Environmental and Industrial Biotechnology: Biotechnology of Thermophiles. Springer Netherlands. 2013. p. 429-457 https://doi.org/10.1007/978-94-007-5899-5_17
Ishino, Sonoko ; Ishino, Yoshizumi. / DNA polymerases and DNA ligases. Thermophilic Microbes in Environmental and Industrial Biotechnology: Biotechnology of Thermophiles. Springer Netherlands, 2013. pp. 429-457
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