DNA sensors using a ferrocene-oligonucleotide conjugate

Masamichi Nakayama, Toshihiro Ihara, Koji Nakano, Mizuo Maeda

Research output: Contribution to journalArticle

88 Citations (Scopus)

Abstract

Using the redox-active DNA conjugate (ferrocene-modified oligonucleotide 12 mer) as a probe, the novel electrochemical gene sensor, which is sensitive, convenient, and not relying on radio isotope, has been developed. Oligonucleotide (16 met) complementary to the target (19 met) was immobilized onto gold electrode through the specific chemisorption of successive phosphorothioates which were introduced into 5′-end of the oligonucleotide. The sequence of the conjugate was designed to be also complementary to another site of the target. Therefore, the conjugate and the oligonucleotide anchoring on the electrode formed a sandwich-type ternary complex with a target DNA to give electric currents based on the ferrocene oxidation. By using this system, we have distinguished the mutant that has one base substitution from the fully complementary target.

Original languageEnglish
Pages (from-to)857-866
Number of pages10
JournalTalanta
Volume56
Issue number5
DOIs
Publication statusPublished - Apr 1 2002

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

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