DNA separation by cholesterol-bearing pullulan nanogels

Keisuke Kondo, Noritada Kaji, Sayaka Toita, Yukihiro Okamoto, Manabu Tokeshi, Kazunari Akiyoshi, Yoshinobu Baba

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

We present an application of a novel DNA separation matrix, cholesterol-bearing pullulan (CHP) nanogels, for microchip electrophoresis. The solution of the CHP showed a unique phase transition around 30 mg/ml and formed gel phase over this critical concentration. This gel phase consists of the weak hydrophobic interactions between the cholesterols could be easily deformed by external forces, and thus, loading process of the CHP nanogels into microchannels became easier. The high concentration of the CHP nanogels provided excellent resolutions especially for small DNA fragments from 100 to 1500 bp. The separation mechanism was discussed based on Ogston and Reptation models which had developed in gels or polymer solutions. The result of a single molecule imaging gave us an insight of the separation mechanism and the nanogel structures as well.

Original languageEnglish
Article number032210
JournalBiomicrofluidics
Volume4
Issue number3
DOIs
Publication statusPublished - Jan 1 2010
Externally publishedYes

Fingerprint

Bearings (structural)
Cholesterol
cholesterol
DNA
deoxyribonucleic acid
Gels
gels
Microchip Electrophoresis
Phase Transition
Polymer solutions
electrophoresis
microchannels
Microchannels
Electrophoresis
Hydrophobic and Hydrophilic Interactions
Polymers
Phase transitions
fragments
pullulan
NanoGel

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Materials Science(all)
  • Genetics
  • Condensed Matter Physics
  • Physical and Theoretical Chemistry

Cite this

Kondo, K., Kaji, N., Toita, S., Okamoto, Y., Tokeshi, M., Akiyoshi, K., & Baba, Y. (2010). DNA separation by cholesterol-bearing pullulan nanogels. Biomicrofluidics, 4(3), [032210]. https://doi.org/10.1063/1.3479997

DNA separation by cholesterol-bearing pullulan nanogels. / Kondo, Keisuke; Kaji, Noritada; Toita, Sayaka; Okamoto, Yukihiro; Tokeshi, Manabu; Akiyoshi, Kazunari; Baba, Yoshinobu.

In: Biomicrofluidics, Vol. 4, No. 3, 032210, 01.01.2010.

Research output: Contribution to journalArticle

Kondo, K, Kaji, N, Toita, S, Okamoto, Y, Tokeshi, M, Akiyoshi, K & Baba, Y 2010, 'DNA separation by cholesterol-bearing pullulan nanogels', Biomicrofluidics, vol. 4, no. 3, 032210. https://doi.org/10.1063/1.3479997
Kondo K, Kaji N, Toita S, Okamoto Y, Tokeshi M, Akiyoshi K et al. DNA separation by cholesterol-bearing pullulan nanogels. Biomicrofluidics. 2010 Jan 1;4(3). 032210. https://doi.org/10.1063/1.3479997
Kondo, Keisuke ; Kaji, Noritada ; Toita, Sayaka ; Okamoto, Yukihiro ; Tokeshi, Manabu ; Akiyoshi, Kazunari ; Baba, Yoshinobu. / DNA separation by cholesterol-bearing pullulan nanogels. In: Biomicrofluidics. 2010 ; Vol. 4, No. 3.
@article{484e5101b7cd4d41947a8cd1350bc3d9,
title = "DNA separation by cholesterol-bearing pullulan nanogels",
abstract = "We present an application of a novel DNA separation matrix, cholesterol-bearing pullulan (CHP) nanogels, for microchip electrophoresis. The solution of the CHP showed a unique phase transition around 30 mg/ml and formed gel phase over this critical concentration. This gel phase consists of the weak hydrophobic interactions between the cholesterols could be easily deformed by external forces, and thus, loading process of the CHP nanogels into microchannels became easier. The high concentration of the CHP nanogels provided excellent resolutions especially for small DNA fragments from 100 to 1500 bp. The separation mechanism was discussed based on Ogston and Reptation models which had developed in gels or polymer solutions. The result of a single molecule imaging gave us an insight of the separation mechanism and the nanogel structures as well.",
author = "Keisuke Kondo and Noritada Kaji and Sayaka Toita and Yukihiro Okamoto and Manabu Tokeshi and Kazunari Akiyoshi and Yoshinobu Baba",
year = "2010",
month = "1",
day = "1",
doi = "10.1063/1.3479997",
language = "English",
volume = "4",
journal = "Biomicrofluidics",
issn = "1932-1058",
publisher = "American Institute of Physics Publising LLC",
number = "3",

}

TY - JOUR

T1 - DNA separation by cholesterol-bearing pullulan nanogels

AU - Kondo, Keisuke

AU - Kaji, Noritada

AU - Toita, Sayaka

AU - Okamoto, Yukihiro

AU - Tokeshi, Manabu

AU - Akiyoshi, Kazunari

AU - Baba, Yoshinobu

PY - 2010/1/1

Y1 - 2010/1/1

N2 - We present an application of a novel DNA separation matrix, cholesterol-bearing pullulan (CHP) nanogels, for microchip electrophoresis. The solution of the CHP showed a unique phase transition around 30 mg/ml and formed gel phase over this critical concentration. This gel phase consists of the weak hydrophobic interactions between the cholesterols could be easily deformed by external forces, and thus, loading process of the CHP nanogels into microchannels became easier. The high concentration of the CHP nanogels provided excellent resolutions especially for small DNA fragments from 100 to 1500 bp. The separation mechanism was discussed based on Ogston and Reptation models which had developed in gels or polymer solutions. The result of a single molecule imaging gave us an insight of the separation mechanism and the nanogel structures as well.

AB - We present an application of a novel DNA separation matrix, cholesterol-bearing pullulan (CHP) nanogels, for microchip electrophoresis. The solution of the CHP showed a unique phase transition around 30 mg/ml and formed gel phase over this critical concentration. This gel phase consists of the weak hydrophobic interactions between the cholesterols could be easily deformed by external forces, and thus, loading process of the CHP nanogels into microchannels became easier. The high concentration of the CHP nanogels provided excellent resolutions especially for small DNA fragments from 100 to 1500 bp. The separation mechanism was discussed based on Ogston and Reptation models which had developed in gels or polymer solutions. The result of a single molecule imaging gave us an insight of the separation mechanism and the nanogel structures as well.

UR - http://www.scopus.com/inward/record.url?scp=77957884595&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77957884595&partnerID=8YFLogxK

U2 - 10.1063/1.3479997

DO - 10.1063/1.3479997

M3 - Article

C2 - 21045931

AN - SCOPUS:77957884595

VL - 4

JO - Biomicrofluidics

JF - Biomicrofluidics

SN - 1932-1058

IS - 3

M1 - 032210

ER -