DNA separation by cholesterol-bearing pullulan nanogels

Keisuke Kondo, Noritada Kaji, Sayaka Toita, Yukihiro Okamoto, Manabu Tokeshi, Kazunari Akiyoshi, Yoshinobu Baba

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

We present an application of a novel DNA separation matrix, cholesterol-bearing pullulan (CHP) nanogels, for microchip electrophoresis. The solution of the CHP showed a unique phase transition around 30 mg/ml and formed gel phase over this critical concentration. This gel phase consists of the weak hydrophobic interactions between the cholesterols could be easily deformed by external forces, and thus, loading process of the CHP nanogels into microchannels became easier. The high concentration of the CHP nanogels provided excellent resolutions especially for small DNA fragments from 100 to 1500 bp. The separation mechanism was discussed based on Ogston and Reptation models which had developed in gels or polymer solutions. The result of a single molecule imaging gave us an insight of the separation mechanism and the nanogel structures as well.

Original languageEnglish
Article number032210
JournalBiomicrofluidics
Volume4
Issue number3
DOIs
Publication statusPublished - 2010

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Materials Science(all)
  • Genetics
  • Condensed Matter Physics
  • Physical and Theoretical Chemistry

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    Kondo, K., Kaji, N., Toita, S., Okamoto, Y., Tokeshi, M., Akiyoshi, K., & Baba, Y. (2010). DNA separation by cholesterol-bearing pullulan nanogels. Biomicrofluidics, 4(3), [032210]. https://doi.org/10.1063/1.3479997