DNA sequence analysis of a chromosomal aberration in irradiation induced burnt (Bu) mutant of Bombyx mori

Tsuguru Fujii, Maki Kubo, Seigou Kuwazaki, Kimiko Yamamoto, Akio Ohnuma, Yutaka Banno, Toru Shimada

Research output: Contribution to journalArticlepeer-review

Abstract

The radiation-induced mutations burnt (Bu) and non-preference A4 (Np-A4) map to chromosome 11 in Bom-byx mori. Bu causes irregularly shaped fifth to eighth larval segments, whereas Np-A4 causes one of the po-lyphagous phenotypes, i.e., feeding on non-mulberry leaves. Homozygotes and trans heterozygotes of these mutations die during embryogenesis, suggesting that the lethality associated with Bu and Np-A4 is allelic. Genetic studies of the Bu and Np-A4 mutation would provide a better understanding of the embryogenesis, mor-phogenesis, and feeding behavior of B. mori. Here we conducted a molecular analysis of the Bu locus. In our crossing experiments, larvae carrying both the Bu and Np-A4 mutations appeared, although they were hypoth-esized to die during embryogenesis. A detailed analysis of the survivors resulted in the identification of a breakage event on chromosome 11. Moreover, it was revealed that the survivors exhibited partial trisomy. Using DNA sequence analysis, the breakpoint in chromosome 11 was narrowed to an intron of Bmzfh3. Embryonic lethality associated with the breakage of the chromosome 11 is discussed.

Original languageEnglish
Pages (from-to)41-50
Number of pages10
JournalJournal of Insect Biotechnology and Sericology
Volume91
Issue number3
DOIs
Publication statusPublished - 2022

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Business, Management and Accounting(all)
  • Agricultural and Biological Sciences(all)
  • Insect Science
  • Industrial and Manufacturing Engineering

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