DNA strand breaks (comet assay) and embryo development effects in grass shrimp (Palaemonetes pugio) embryos after exposure to genotoxicants

R. Lee, G. B. Kim, K. A. Maruya, S. A. Steinert, Yuji Oshima

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Grass shrimp embryos develop in egg sacs (stages 1-10) attached to the female for 14-20 days after which they 'hatch' from the egg sacs into a swimming zoea stage (stage 11). Until they emerge from the egg sacs, embryos depend on lipids and lipovitellin stored within the egg. The percent of embryos which hatch after exposure to toxicants relative to controls was the basis of an embryo development assay. Exposure of embryos to chromium(III) chloride, sodium chromate, mercuric chloride, and 2-methyl-1,2-naphthoquinone (MNQ) resulted in a reduced hatching rate. In addition to effects on embryo development, DNA strand damage tests were carried out on contaminant-exposed embryos, using the single-cell electrophoresis method often referred to as comet assay. Development of stage 4 embryos was more affected by MNQ exposure than stage 7 embryos. The hatching rates of stages 4 and 7 embryos exposed to MNQ (172 μg/l) were 0 and 90%, respectively. DNA strand damage, measured as DNA tail moments, were 3.4 and 4.4, respectively. Thus, exposure of an early embryo stage to MNQ prevented full embryo development while development of later embryo stages was not affected. It may be that the DNA repair systems are more efficient in later embryo stages than in early stages and thus DNA damaged in the early stages affects development. Copyright (C) 2000 Elsevier Science Ltd.

Original languageEnglish
Pages (from-to)553-557
Number of pages5
JournalMarine Environmental Research
Volume50
Issue number1-5
DOIs
Publication statusPublished - Nov 14 2000

Fingerprint

Palaemonetes pugio
Palaemonetes
comet
embryo
Assays
embryo (animal)
DNA
embryogenesis
grass
assay
assays
Hatches
naphthoquinones
Chromates
Sodium chloride
Electrophoresis
egg
Lipids
Chromium
Repair

All Science Journal Classification (ASJC) codes

  • Aquatic Science
  • Oceanography
  • Environmental Science(all)

Cite this

DNA strand breaks (comet assay) and embryo development effects in grass shrimp (Palaemonetes pugio) embryos after exposure to genotoxicants. / Lee, R.; Kim, G. B.; Maruya, K. A.; Steinert, S. A.; Oshima, Yuji.

In: Marine Environmental Research, Vol. 50, No. 1-5, 14.11.2000, p. 553-557.

Research output: Contribution to journalArticle

@article{0c51692944ae4ba0bd8c3fffa181120c,
title = "DNA strand breaks (comet assay) and embryo development effects in grass shrimp (Palaemonetes pugio) embryos after exposure to genotoxicants",
abstract = "Grass shrimp embryos develop in egg sacs (stages 1-10) attached to the female for 14-20 days after which they 'hatch' from the egg sacs into a swimming zoea stage (stage 11). Until they emerge from the egg sacs, embryos depend on lipids and lipovitellin stored within the egg. The percent of embryos which hatch after exposure to toxicants relative to controls was the basis of an embryo development assay. Exposure of embryos to chromium(III) chloride, sodium chromate, mercuric chloride, and 2-methyl-1,2-naphthoquinone (MNQ) resulted in a reduced hatching rate. In addition to effects on embryo development, DNA strand damage tests were carried out on contaminant-exposed embryos, using the single-cell electrophoresis method often referred to as comet assay. Development of stage 4 embryos was more affected by MNQ exposure than stage 7 embryos. The hatching rates of stages 4 and 7 embryos exposed to MNQ (172 μg/l) were 0 and 90{\%}, respectively. DNA strand damage, measured as DNA tail moments, were 3.4 and 4.4, respectively. Thus, exposure of an early embryo stage to MNQ prevented full embryo development while development of later embryo stages was not affected. It may be that the DNA repair systems are more efficient in later embryo stages than in early stages and thus DNA damaged in the early stages affects development. Copyright (C) 2000 Elsevier Science Ltd.",
author = "R. Lee and Kim, {G. B.} and Maruya, {K. A.} and Steinert, {S. A.} and Yuji Oshima",
year = "2000",
month = "11",
day = "14",
doi = "10.1016/S0141-1136(00)00110-0",
language = "English",
volume = "50",
pages = "553--557",
journal = "Marine Environmental Research",
issn = "0141-1136",
publisher = "Elsevier BV",
number = "1-5",

}

TY - JOUR

T1 - DNA strand breaks (comet assay) and embryo development effects in grass shrimp (Palaemonetes pugio) embryos after exposure to genotoxicants

AU - Lee, R.

AU - Kim, G. B.

AU - Maruya, K. A.

AU - Steinert, S. A.

AU - Oshima, Yuji

PY - 2000/11/14

Y1 - 2000/11/14

N2 - Grass shrimp embryos develop in egg sacs (stages 1-10) attached to the female for 14-20 days after which they 'hatch' from the egg sacs into a swimming zoea stage (stage 11). Until they emerge from the egg sacs, embryos depend on lipids and lipovitellin stored within the egg. The percent of embryos which hatch after exposure to toxicants relative to controls was the basis of an embryo development assay. Exposure of embryos to chromium(III) chloride, sodium chromate, mercuric chloride, and 2-methyl-1,2-naphthoquinone (MNQ) resulted in a reduced hatching rate. In addition to effects on embryo development, DNA strand damage tests were carried out on contaminant-exposed embryos, using the single-cell electrophoresis method often referred to as comet assay. Development of stage 4 embryos was more affected by MNQ exposure than stage 7 embryos. The hatching rates of stages 4 and 7 embryos exposed to MNQ (172 μg/l) were 0 and 90%, respectively. DNA strand damage, measured as DNA tail moments, were 3.4 and 4.4, respectively. Thus, exposure of an early embryo stage to MNQ prevented full embryo development while development of later embryo stages was not affected. It may be that the DNA repair systems are more efficient in later embryo stages than in early stages and thus DNA damaged in the early stages affects development. Copyright (C) 2000 Elsevier Science Ltd.

AB - Grass shrimp embryos develop in egg sacs (stages 1-10) attached to the female for 14-20 days after which they 'hatch' from the egg sacs into a swimming zoea stage (stage 11). Until they emerge from the egg sacs, embryos depend on lipids and lipovitellin stored within the egg. The percent of embryos which hatch after exposure to toxicants relative to controls was the basis of an embryo development assay. Exposure of embryos to chromium(III) chloride, sodium chromate, mercuric chloride, and 2-methyl-1,2-naphthoquinone (MNQ) resulted in a reduced hatching rate. In addition to effects on embryo development, DNA strand damage tests were carried out on contaminant-exposed embryos, using the single-cell electrophoresis method often referred to as comet assay. Development of stage 4 embryos was more affected by MNQ exposure than stage 7 embryos. The hatching rates of stages 4 and 7 embryos exposed to MNQ (172 μg/l) were 0 and 90%, respectively. DNA strand damage, measured as DNA tail moments, were 3.4 and 4.4, respectively. Thus, exposure of an early embryo stage to MNQ prevented full embryo development while development of later embryo stages was not affected. It may be that the DNA repair systems are more efficient in later embryo stages than in early stages and thus DNA damaged in the early stages affects development. Copyright (C) 2000 Elsevier Science Ltd.

UR - http://www.scopus.com/inward/record.url?scp=0033734946&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033734946&partnerID=8YFLogxK

U2 - 10.1016/S0141-1136(00)00110-0

DO - 10.1016/S0141-1136(00)00110-0

M3 - Article

C2 - 11460748

AN - SCOPUS:0033734946

VL - 50

SP - 553

EP - 557

JO - Marine Environmental Research

JF - Marine Environmental Research

SN - 0141-1136

IS - 1-5

ER -