DNA stretching method using a flow induced by laser irradiation under AC electric field

Michihiko Nakano, Kazunori Takashima, Shinji Katsura, Akira Mizuno

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

A micro-vortex is generated around an infrared laser focus where an intense AC electric field is applied. We used this opto-electrostatic micro-vortex to stretch individual long DNA molecules. When T4-phage DNA molecules were introduced into the opto-electrostatic micro-vortex, they were stretched around the laser focus. In addition, it was possible to keep it in stretching form for more than 30 sec. Using this method, length of DNA molecules can be measured without fixing to a substrate.

Original languageEnglish
Title of host publicationMicro Total Analysis Systems - Proceedings of MicroTAS 2006 Conference: 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherJapan Academic Association Inc
Pages1525-1527
Number of pages3
Publication statusPublished - 2006
Externally publishedYes
Event10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006 - Tokyo, Japan
Duration: Nov 5 2006Nov 9 2006

Other

Other10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006
CountryJapan
CityTokyo
Period11/5/0611/9/06

Fingerprint

Laser beam effects
Stretching
Vortex flow
DNA
Electric fields
Molecules
Electrostatics
Bacteriophages
Infrared lasers
Lasers
Substrates

All Science Journal Classification (ASJC) codes

  • Chemical Engineering (miscellaneous)
  • Bioengineering

Cite this

Nakano, M., Takashima, K., Katsura, S., & Mizuno, A. (2006). DNA stretching method using a flow induced by laser irradiation under AC electric field. In Micro Total Analysis Systems - Proceedings of MicroTAS 2006 Conference: 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 1525-1527). Japan Academic Association Inc.

DNA stretching method using a flow induced by laser irradiation under AC electric field. / Nakano, Michihiko; Takashima, Kazunori; Katsura, Shinji; Mizuno, Akira.

Micro Total Analysis Systems - Proceedings of MicroTAS 2006 Conference: 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Japan Academic Association Inc, 2006. p. 1525-1527.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Nakano, M, Takashima, K, Katsura, S & Mizuno, A 2006, DNA stretching method using a flow induced by laser irradiation under AC electric field. in Micro Total Analysis Systems - Proceedings of MicroTAS 2006 Conference: 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Japan Academic Association Inc, pp. 1525-1527, 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006, Tokyo, Japan, 11/5/06.
Nakano M, Takashima K, Katsura S, Mizuno A. DNA stretching method using a flow induced by laser irradiation under AC electric field. In Micro Total Analysis Systems - Proceedings of MicroTAS 2006 Conference: 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Japan Academic Association Inc. 2006. p. 1525-1527
Nakano, Michihiko ; Takashima, Kazunori ; Katsura, Shinji ; Mizuno, Akira. / DNA stretching method using a flow induced by laser irradiation under AC electric field. Micro Total Analysis Systems - Proceedings of MicroTAS 2006 Conference: 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Japan Academic Association Inc, 2006. pp. 1525-1527
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