DNA typing of HLA class II genes in Japanese patients with dilated cardiomyopathy

Hirofumi Nishi, Yoshinori Koga, Takeshi Koyanagi, Haruhito Harada, Tsutomu Imaizumi, Hironori Toshima, Takehiko Sasazuki, Akinori Kimura

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

HLA class II genes (DRB, DQA, DQB, DPA, and DPB) were typed at the DNA level using polymerase chain reaction/sequence-specfic oligonnucleotide probe analysis in 78 unrelated patients with DCM and 336 unrelated healthy controls to elucidate the HLA alleles or HLA haplotypes associated with DCM. The frequencies of DRB * 1401 (14.4% v 4.5%, RR = 3.90, P<0.0005, Pc<0.03), DQB1*0503 (14.1% v 5.4%, RR=2.93, P<0.007) and DRB1*1401-DQB1*0503 haplotype (11.5% v 1.5%, RR = 8.24, P<0.00001, Pc<0.01) were increased in the DCM patients. The frequency of HLA-DRB1*1101 (9.0% v 3.0%, RR = 3.26, P<0.02) also was increased in the patients. In addition, the frequencies of DQB1*0604 and DPB1*0401 were increased in the DRB1*1401 and DRB1*1101 negative patients. In contrast, the frequencies of DQB1*0303 (19.2% v 30.7%, RR = 0.55, P<0.05) and DRB1*0901-DQB1*0.03 haplotype (16.7% v 29.8%, RR = 0.49, P<0.02) were decreased in the DCM group. Disease susceptibility to DCM in the Japanese population, thus, may be controlled in part by a gene (or genes) in close linkage disequilibrium with DRB1*1401-DQB1*0503, DRB1*1101-DQB1*0301, and DQB1*0604-DPB1*0401 haplotypes, while the resistance to DCM may be associated with the DRB1*0901-DQB1*0303 haplotype.

Original languageEnglish
Pages (from-to)2385-2392
Number of pages8
JournalJournal of Molecular and Cellular Cardiology
Volume27
Issue number10
DOIs
Publication statusPublished - Oct 1995

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cardiology and Cardiovascular Medicine

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