Abstract
Apoptosis and necrosis, two major forms of cell death, can be distinguished morphologically and biochemically. Internucleosomal DNA fragmentation (INDF) is a biochemical hallmark of apoptosis, and caspase-activated DNase (CAD), also known as DNA fragmentation factor 40 kDa (DFF40), is one of the major effector endonucleases. DNase γ, a Mg2+/Ca2+-dependent endonuclease, is also known to generate INDF but its role among other apoptosis-associated endonucleases in cell death is unclear. Here we show that (i) INDF occurs even during necrosis in cell lines, primary cells, and in tissues of mice in vivo, and (ii) DNase c, but not CAD, is the effector endonuclease for INDF in cells undergoing necrosis. These results document a previously unappreciated role for INDF in necrosis and define its molecular basis. Copyright:
| Original language | English |
|---|---|
| Article number | e80223 |
| Journal | PloS one |
| Volume | 8 |
| Issue number | 12 |
| DOIs | |
| Publication status | Published - Dec 2 2013 |
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All Science Journal Classification (ASJC) codes
- Biochemistry, Genetics and Molecular Biology(all)
- Agricultural and Biological Sciences(all)
- General
Cite this
DNase γ is the effector endonuclease for internucleosomal DNA fragmentation in necrosis. / Mizuta, Ryushin; Araki, Shinsuke; Furukawa, Makoto; Furukawa, Yuki; Ebara, Syota; Shiokawa, Daisuke; Hayashi, Katsuhiko; Tanuma, Sei Ichi; Kitamura, Daisuke.
In: PloS one, Vol. 8, No. 12, e80223, 02.12.2013.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - DNase γ is the effector endonuclease for internucleosomal DNA fragmentation in necrosis
AU - Mizuta, Ryushin
AU - Araki, Shinsuke
AU - Furukawa, Makoto
AU - Furukawa, Yuki
AU - Ebara, Syota
AU - Shiokawa, Daisuke
AU - Hayashi, Katsuhiko
AU - Tanuma, Sei Ichi
AU - Kitamura, Daisuke
PY - 2013/12/2
Y1 - 2013/12/2
N2 - Apoptosis and necrosis, two major forms of cell death, can be distinguished morphologically and biochemically. Internucleosomal DNA fragmentation (INDF) is a biochemical hallmark of apoptosis, and caspase-activated DNase (CAD), also known as DNA fragmentation factor 40 kDa (DFF40), is one of the major effector endonucleases. DNase γ, a Mg2+/Ca2+-dependent endonuclease, is also known to generate INDF but its role among other apoptosis-associated endonucleases in cell death is unclear. Here we show that (i) INDF occurs even during necrosis in cell lines, primary cells, and in tissues of mice in vivo, and (ii) DNase c, but not CAD, is the effector endonuclease for INDF in cells undergoing necrosis. These results document a previously unappreciated role for INDF in necrosis and define its molecular basis. Copyright:
AB - Apoptosis and necrosis, two major forms of cell death, can be distinguished morphologically and biochemically. Internucleosomal DNA fragmentation (INDF) is a biochemical hallmark of apoptosis, and caspase-activated DNase (CAD), also known as DNA fragmentation factor 40 kDa (DFF40), is one of the major effector endonucleases. DNase γ, a Mg2+/Ca2+-dependent endonuclease, is also known to generate INDF but its role among other apoptosis-associated endonucleases in cell death is unclear. Here we show that (i) INDF occurs even during necrosis in cell lines, primary cells, and in tissues of mice in vivo, and (ii) DNase c, but not CAD, is the effector endonuclease for INDF in cells undergoing necrosis. These results document a previously unappreciated role for INDF in necrosis and define its molecular basis. Copyright:
UR - http://www.scopus.com/inward/record.url?scp=84891591519&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84891591519&partnerID=8YFLogxK
U2 - 10.1371/journal.pone.0080223
DO - 10.1371/journal.pone.0080223
M3 - Article
C2 - 24312463
AN - SCOPUS:84891591519
VL - 8
JO - PLoS One
JF - PLoS One
SN - 1932-6203
IS - 12
M1 - e80223
ER -