DNase γ is the effector endonuclease for internucleosomal DNA fragmentation in necrosis

Ryushin Mizuta; Shinsuke Araki; Makoto Furukawa; Yuki Furukawa; Syota Ebara; Daisuke Shiokawa; Katsuhiko Hayashi; Sei Ichi Tanuma; Daisuke Kitamura

doi:10.1371/journal.pone.0080223

DNase γ is the effector endonuclease for internucleosomal DNA fragmentation in necrosis

Ryushin Mizuta, Shinsuke Araki, Makoto Furukawa, Yuki Furukawa, Syota Ebara, Daisuke Shiokawa, Katsuhiko Hayashi, Sei Ichi Tanuma, Daisuke Kitamura

Stem Cell Biology and Medicine

Research output: Contribution to journal › Article › peer-review

25 Citations (Scopus)

Abstract

Apoptosis and necrosis, two major forms of cell death, can be distinguished morphologically and biochemically. Internucleosomal DNA fragmentation (INDF) is a biochemical hallmark of apoptosis, and caspase-activated DNase (CAD), also known as DNA fragmentation factor 40 kDa (DFF40), is one of the major effector endonucleases. DNase γ, a Mg²⁺/Ca²⁺-dependent endonuclease, is also known to generate INDF but its role among other apoptosis-associated endonucleases in cell death is unclear. Here we show that (i) INDF occurs even during necrosis in cell lines, primary cells, and in tissues of mice in vivo, and (ii) DNase c, but not CAD, is the effector endonuclease for INDF in cells undergoing necrosis. These results document a previously unappreciated role for INDF in necrosis and define its molecular basis. Copyright:

Original language	English
Article number	e80223
Journal	PloS one
Volume	8
Issue number	12
DOIs	https://doi.org/10.1371/journal.pone.0080223
Publication status	Published - Dec 2 2013

All Science Journal Classification (ASJC) codes

Biochemistry, Genetics and Molecular Biology(all)
Agricultural and Biological Sciences(all)
General

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title = "DNase γ is the effector endonuclease for internucleosomal DNA fragmentation in necrosis",

abstract = "Apoptosis and necrosis, two major forms of cell death, can be distinguished morphologically and biochemically. Internucleosomal DNA fragmentation (INDF) is a biochemical hallmark of apoptosis, and caspase-activated DNase (CAD), also known as DNA fragmentation factor 40 kDa (DFF40), is one of the major effector endonucleases. DNase γ, a Mg2+/Ca2+-dependent endonuclease, is also known to generate INDF but its role among other apoptosis-associated endonucleases in cell death is unclear. Here we show that (i) INDF occurs even during necrosis in cell lines, primary cells, and in tissues of mice in vivo, and (ii) DNase c, but not CAD, is the effector endonuclease for INDF in cells undergoing necrosis. These results document a previously unappreciated role for INDF in necrosis and define its molecular basis. Copyright:",

author = "Ryushin Mizuta and Shinsuke Araki and Makoto Furukawa and Yuki Furukawa and Syota Ebara and Daisuke Shiokawa and Katsuhiko Hayashi and Tanuma, {Sei Ichi} and Daisuke Kitamura",

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AU - Mizuta, Ryushin

AU - Araki, Shinsuke

AU - Furukawa, Makoto

AU - Furukawa, Yuki

AU - Ebara, Syota

AU - Shiokawa, Daisuke

AU - Hayashi, Katsuhiko

AU - Tanuma, Sei Ichi

AU - Kitamura, Daisuke

PY - 2013/12/2

Y1 - 2013/12/2

N2 - Apoptosis and necrosis, two major forms of cell death, can be distinguished morphologically and biochemically. Internucleosomal DNA fragmentation (INDF) is a biochemical hallmark of apoptosis, and caspase-activated DNase (CAD), also known as DNA fragmentation factor 40 kDa (DFF40), is one of the major effector endonucleases. DNase γ, a Mg2+/Ca2+-dependent endonuclease, is also known to generate INDF but its role among other apoptosis-associated endonucleases in cell death is unclear. Here we show that (i) INDF occurs even during necrosis in cell lines, primary cells, and in tissues of mice in vivo, and (ii) DNase c, but not CAD, is the effector endonuclease for INDF in cells undergoing necrosis. These results document a previously unappreciated role for INDF in necrosis and define its molecular basis. Copyright:

AB - Apoptosis and necrosis, two major forms of cell death, can be distinguished morphologically and biochemically. Internucleosomal DNA fragmentation (INDF) is a biochemical hallmark of apoptosis, and caspase-activated DNase (CAD), also known as DNA fragmentation factor 40 kDa (DFF40), is one of the major effector endonucleases. DNase γ, a Mg2+/Ca2+-dependent endonuclease, is also known to generate INDF but its role among other apoptosis-associated endonucleases in cell death is unclear. Here we show that (i) INDF occurs even during necrosis in cell lines, primary cells, and in tissues of mice in vivo, and (ii) DNase c, but not CAD, is the effector endonuclease for INDF in cells undergoing necrosis. These results document a previously unappreciated role for INDF in necrosis and define its molecular basis. Copyright:

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