Abstract
Objective. To verify whether hypertension confers a hypercoagulable state in a hypertensive animal model. Design. The parameters of blood coagulation were compared between stroke-prone spontaneously hypertensive rats (SHR-SP) and Wistar-Kyoto (WKY) rats. Each rat group consisted of a younger subgroup at 8-12 weeks old (n = 12) and an older subgroup at 16-20 weeks old (n = 12). Methods. Prothrombin time (PT), activated partial thromboplastin time (APTT), fluorogenic PT, fibrinogen, fibrin/fibrinogen degradation products (FDP), thrombin-anti-thrombin III complex (TAT), factor Xa activity, antithrombin III (AT-III), tissue factor pathway inhibitor (TFPI), protein C and C1 inhibitor were measured in both rat groups. Results. There was no significant difference in FDP and TAT levels between SHR-SP and WKY rats even at 16-20 weeks when SHR-SP developed severe hypertensive vascular lesions. Contrary to expectations, fluorogenic PT and factor Xa activity were significantly lower in SHR-SP than in WKY rats. While there was no significant difference in AT-III, TFPI and protein C activities between SHR-SP and WKY rats, C1 inhibitor activity was significantly higher in SHR-SP than in WKY rats. The elevated C1 inhibitor activity was inversely correlated with the reduced factor Xa activity. Gel-filtered fractionated plasma with C1 inhibitor activity had an inhibitory effect on the purified rat factor Xa, and immunodepletion of C1 inhibitor from the fractionated plasma attenuated the inhibitory effect. Conclusion. These results suggest that SHR-SP get into a hypocoagulable state rather than a hypercoagulable state, and that the reduction of factor Xa activity in SHR-SP may be related to the elevation of C1 inhibitor activity. (C) Lippincott Williams and Wilkins.
| Original language | English |
|---|---|
| Pages (from-to) | 901-909 |
| Number of pages | 9 |
| Journal | Journal of hypertension |
| Volume | 18 |
| Issue number | 7 |
| DOIs | |
| Publication status | Published - Jan 1 2000 |
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All Science Journal Classification (ASJC) codes
- Internal Medicine
- Physiology
- Cardiology and Cardiovascular Medicine
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Does hypertension confer a hypercoagulable state in stroke-prone spontaneously hypertensive rats? / Abumiya, Takeo; Sakata, Toshiyuki; Enjyoji, Kei Ichi; Kato, Hisao; Kawai, Jun; Suzuki, Tsuneyuki; Masuda, Junichi; Sasaguri, Toshiyuki; Ogata, Jun.
In: Journal of hypertension, Vol. 18, No. 7, 01.01.2000, p. 901-909.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Does hypertension confer a hypercoagulable state in stroke-prone spontaneously hypertensive rats?
AU - Abumiya, Takeo
AU - Sakata, Toshiyuki
AU - Enjyoji, Kei Ichi
AU - Kato, Hisao
AU - Kawai, Jun
AU - Suzuki, Tsuneyuki
AU - Masuda, Junichi
AU - Sasaguri, Toshiyuki
AU - Ogata, Jun
PY - 2000/1/1
Y1 - 2000/1/1
N2 - Objective. To verify whether hypertension confers a hypercoagulable state in a hypertensive animal model. Design. The parameters of blood coagulation were compared between stroke-prone spontaneously hypertensive rats (SHR-SP) and Wistar-Kyoto (WKY) rats. Each rat group consisted of a younger subgroup at 8-12 weeks old (n = 12) and an older subgroup at 16-20 weeks old (n = 12). Methods. Prothrombin time (PT), activated partial thromboplastin time (APTT), fluorogenic PT, fibrinogen, fibrin/fibrinogen degradation products (FDP), thrombin-anti-thrombin III complex (TAT), factor Xa activity, antithrombin III (AT-III), tissue factor pathway inhibitor (TFPI), protein C and C1 inhibitor were measured in both rat groups. Results. There was no significant difference in FDP and TAT levels between SHR-SP and WKY rats even at 16-20 weeks when SHR-SP developed severe hypertensive vascular lesions. Contrary to expectations, fluorogenic PT and factor Xa activity were significantly lower in SHR-SP than in WKY rats. While there was no significant difference in AT-III, TFPI and protein C activities between SHR-SP and WKY rats, C1 inhibitor activity was significantly higher in SHR-SP than in WKY rats. The elevated C1 inhibitor activity was inversely correlated with the reduced factor Xa activity. Gel-filtered fractionated plasma with C1 inhibitor activity had an inhibitory effect on the purified rat factor Xa, and immunodepletion of C1 inhibitor from the fractionated plasma attenuated the inhibitory effect. Conclusion. These results suggest that SHR-SP get into a hypocoagulable state rather than a hypercoagulable state, and that the reduction of factor Xa activity in SHR-SP may be related to the elevation of C1 inhibitor activity. (C) Lippincott Williams and Wilkins.
AB - Objective. To verify whether hypertension confers a hypercoagulable state in a hypertensive animal model. Design. The parameters of blood coagulation were compared between stroke-prone spontaneously hypertensive rats (SHR-SP) and Wistar-Kyoto (WKY) rats. Each rat group consisted of a younger subgroup at 8-12 weeks old (n = 12) and an older subgroup at 16-20 weeks old (n = 12). Methods. Prothrombin time (PT), activated partial thromboplastin time (APTT), fluorogenic PT, fibrinogen, fibrin/fibrinogen degradation products (FDP), thrombin-anti-thrombin III complex (TAT), factor Xa activity, antithrombin III (AT-III), tissue factor pathway inhibitor (TFPI), protein C and C1 inhibitor were measured in both rat groups. Results. There was no significant difference in FDP and TAT levels between SHR-SP and WKY rats even at 16-20 weeks when SHR-SP developed severe hypertensive vascular lesions. Contrary to expectations, fluorogenic PT and factor Xa activity were significantly lower in SHR-SP than in WKY rats. While there was no significant difference in AT-III, TFPI and protein C activities between SHR-SP and WKY rats, C1 inhibitor activity was significantly higher in SHR-SP than in WKY rats. The elevated C1 inhibitor activity was inversely correlated with the reduced factor Xa activity. Gel-filtered fractionated plasma with C1 inhibitor activity had an inhibitory effect on the purified rat factor Xa, and immunodepletion of C1 inhibitor from the fractionated plasma attenuated the inhibitory effect. Conclusion. These results suggest that SHR-SP get into a hypocoagulable state rather than a hypercoagulable state, and that the reduction of factor Xa activity in SHR-SP may be related to the elevation of C1 inhibitor activity. (C) Lippincott Williams and Wilkins.
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U2 - 10.1097/00004872-200018070-00012
DO - 10.1097/00004872-200018070-00012
M3 - Article
C2 - 10930188
AN - SCOPUS:0033910299
VL - 18
SP - 901
EP - 909
JO - Journal of Hypertension
JF - Journal of Hypertension
SN - 0263-6352
IS - 7
ER -