Does hypertension confer a hypercoagulable state in stroke-prone spontaneously hypertensive rats?

Takeo Abumiya, Toshiyuki Sakata, Kei Ichi Enjyoji, Hisao Kato, Jun Kawai, Tsuneyuki Suzuki, Junichi Masuda, Toshiyuki Sasaguri, Jun Ogata

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Objective. To verify whether hypertension confers a hypercoagulable state in a hypertensive animal model. Design. The parameters of blood coagulation were compared between stroke-prone spontaneously hypertensive rats (SHR-SP) and Wistar-Kyoto (WKY) rats. Each rat group consisted of a younger subgroup at 8-12 weeks old (n = 12) and an older subgroup at 16-20 weeks old (n = 12). Methods. Prothrombin time (PT), activated partial thromboplastin time (APTT), fluorogenic PT, fibrinogen, fibrin/fibrinogen degradation products (FDP), thrombin-anti-thrombin III complex (TAT), factor Xa activity, antithrombin III (AT-III), tissue factor pathway inhibitor (TFPI), protein C and C1 inhibitor were measured in both rat groups. Results. There was no significant difference in FDP and TAT levels between SHR-SP and WKY rats even at 16-20 weeks when SHR-SP developed severe hypertensive vascular lesions. Contrary to expectations, fluorogenic PT and factor Xa activity were significantly lower in SHR-SP than in WKY rats. While there was no significant difference in AT-III, TFPI and protein C activities between SHR-SP and WKY rats, C1 inhibitor activity was significantly higher in SHR-SP than in WKY rats. The elevated C1 inhibitor activity was inversely correlated with the reduced factor Xa activity. Gel-filtered fractionated plasma with C1 inhibitor activity had an inhibitory effect on the purified rat factor Xa, and immunodepletion of C1 inhibitor from the fractionated plasma attenuated the inhibitory effect. Conclusion. These results suggest that SHR-SP get into a hypocoagulable state rather than a hypercoagulable state, and that the reduction of factor Xa activity in SHR-SP may be related to the elevation of C1 inhibitor activity. (C) Lippincott Williams and Wilkins.

Original languageEnglish
Pages (from-to)901-909
Number of pages9
JournalJournal of hypertension
Volume18
Issue number7
DOIs
Publication statusPublished - Jan 1 2000

Fingerprint

Factor Xa
Inbred WKY Rats
Inbred SHR Rats
Stroke
Hypertension
Prothrombin Time
Antithrombin III
Thrombin
Fibrinogen
Fibrin Fibrinogen Degradation Products
Partial Thromboplastin Time
Protein C Inhibitor
Blood Coagulation
Protein C
Blood Vessels
Animal Models
Gels

All Science Journal Classification (ASJC) codes

  • Internal Medicine
  • Physiology
  • Cardiology and Cardiovascular Medicine

Cite this

Abumiya, T., Sakata, T., Enjyoji, K. I., Kato, H., Kawai, J., Suzuki, T., ... Ogata, J. (2000). Does hypertension confer a hypercoagulable state in stroke-prone spontaneously hypertensive rats? Journal of hypertension, 18(7), 901-909. https://doi.org/10.1097/00004872-200018070-00012

Does hypertension confer a hypercoagulable state in stroke-prone spontaneously hypertensive rats? / Abumiya, Takeo; Sakata, Toshiyuki; Enjyoji, Kei Ichi; Kato, Hisao; Kawai, Jun; Suzuki, Tsuneyuki; Masuda, Junichi; Sasaguri, Toshiyuki; Ogata, Jun.

In: Journal of hypertension, Vol. 18, No. 7, 01.01.2000, p. 901-909.

Research output: Contribution to journalArticle

Abumiya, T, Sakata, T, Enjyoji, KI, Kato, H, Kawai, J, Suzuki, T, Masuda, J, Sasaguri, T & Ogata, J 2000, 'Does hypertension confer a hypercoagulable state in stroke-prone spontaneously hypertensive rats?', Journal of hypertension, vol. 18, no. 7, pp. 901-909. https://doi.org/10.1097/00004872-200018070-00012
Abumiya, Takeo ; Sakata, Toshiyuki ; Enjyoji, Kei Ichi ; Kato, Hisao ; Kawai, Jun ; Suzuki, Tsuneyuki ; Masuda, Junichi ; Sasaguri, Toshiyuki ; Ogata, Jun. / Does hypertension confer a hypercoagulable state in stroke-prone spontaneously hypertensive rats?. In: Journal of hypertension. 2000 ; Vol. 18, No. 7. pp. 901-909.
@article{ad45186bfdcb4ca596dff40cac3392db,
title = "Does hypertension confer a hypercoagulable state in stroke-prone spontaneously hypertensive rats?",
abstract = "Objective. To verify whether hypertension confers a hypercoagulable state in a hypertensive animal model. Design. The parameters of blood coagulation were compared between stroke-prone spontaneously hypertensive rats (SHR-SP) and Wistar-Kyoto (WKY) rats. Each rat group consisted of a younger subgroup at 8-12 weeks old (n = 12) and an older subgroup at 16-20 weeks old (n = 12). Methods. Prothrombin time (PT), activated partial thromboplastin time (APTT), fluorogenic PT, fibrinogen, fibrin/fibrinogen degradation products (FDP), thrombin-anti-thrombin III complex (TAT), factor Xa activity, antithrombin III (AT-III), tissue factor pathway inhibitor (TFPI), protein C and C1 inhibitor were measured in both rat groups. Results. There was no significant difference in FDP and TAT levels between SHR-SP and WKY rats even at 16-20 weeks when SHR-SP developed severe hypertensive vascular lesions. Contrary to expectations, fluorogenic PT and factor Xa activity were significantly lower in SHR-SP than in WKY rats. While there was no significant difference in AT-III, TFPI and protein C activities between SHR-SP and WKY rats, C1 inhibitor activity was significantly higher in SHR-SP than in WKY rats. The elevated C1 inhibitor activity was inversely correlated with the reduced factor Xa activity. Gel-filtered fractionated plasma with C1 inhibitor activity had an inhibitory effect on the purified rat factor Xa, and immunodepletion of C1 inhibitor from the fractionated plasma attenuated the inhibitory effect. Conclusion. These results suggest that SHR-SP get into a hypocoagulable state rather than a hypercoagulable state, and that the reduction of factor Xa activity in SHR-SP may be related to the elevation of C1 inhibitor activity. (C) Lippincott Williams and Wilkins.",
author = "Takeo Abumiya and Toshiyuki Sakata and Enjyoji, {Kei Ichi} and Hisao Kato and Jun Kawai and Tsuneyuki Suzuki and Junichi Masuda and Toshiyuki Sasaguri and Jun Ogata",
year = "2000",
month = "1",
day = "1",
doi = "10.1097/00004872-200018070-00012",
language = "English",
volume = "18",
pages = "901--909",
journal = "Journal of Hypertension",
issn = "0263-6352",
publisher = "Lippincott Williams and Wilkins",
number = "7",

}

TY - JOUR

T1 - Does hypertension confer a hypercoagulable state in stroke-prone spontaneously hypertensive rats?

AU - Abumiya, Takeo

AU - Sakata, Toshiyuki

AU - Enjyoji, Kei Ichi

AU - Kato, Hisao

AU - Kawai, Jun

AU - Suzuki, Tsuneyuki

AU - Masuda, Junichi

AU - Sasaguri, Toshiyuki

AU - Ogata, Jun

PY - 2000/1/1

Y1 - 2000/1/1

N2 - Objective. To verify whether hypertension confers a hypercoagulable state in a hypertensive animal model. Design. The parameters of blood coagulation were compared between stroke-prone spontaneously hypertensive rats (SHR-SP) and Wistar-Kyoto (WKY) rats. Each rat group consisted of a younger subgroup at 8-12 weeks old (n = 12) and an older subgroup at 16-20 weeks old (n = 12). Methods. Prothrombin time (PT), activated partial thromboplastin time (APTT), fluorogenic PT, fibrinogen, fibrin/fibrinogen degradation products (FDP), thrombin-anti-thrombin III complex (TAT), factor Xa activity, antithrombin III (AT-III), tissue factor pathway inhibitor (TFPI), protein C and C1 inhibitor were measured in both rat groups. Results. There was no significant difference in FDP and TAT levels between SHR-SP and WKY rats even at 16-20 weeks when SHR-SP developed severe hypertensive vascular lesions. Contrary to expectations, fluorogenic PT and factor Xa activity were significantly lower in SHR-SP than in WKY rats. While there was no significant difference in AT-III, TFPI and protein C activities between SHR-SP and WKY rats, C1 inhibitor activity was significantly higher in SHR-SP than in WKY rats. The elevated C1 inhibitor activity was inversely correlated with the reduced factor Xa activity. Gel-filtered fractionated plasma with C1 inhibitor activity had an inhibitory effect on the purified rat factor Xa, and immunodepletion of C1 inhibitor from the fractionated plasma attenuated the inhibitory effect. Conclusion. These results suggest that SHR-SP get into a hypocoagulable state rather than a hypercoagulable state, and that the reduction of factor Xa activity in SHR-SP may be related to the elevation of C1 inhibitor activity. (C) Lippincott Williams and Wilkins.

AB - Objective. To verify whether hypertension confers a hypercoagulable state in a hypertensive animal model. Design. The parameters of blood coagulation were compared between stroke-prone spontaneously hypertensive rats (SHR-SP) and Wistar-Kyoto (WKY) rats. Each rat group consisted of a younger subgroup at 8-12 weeks old (n = 12) and an older subgroup at 16-20 weeks old (n = 12). Methods. Prothrombin time (PT), activated partial thromboplastin time (APTT), fluorogenic PT, fibrinogen, fibrin/fibrinogen degradation products (FDP), thrombin-anti-thrombin III complex (TAT), factor Xa activity, antithrombin III (AT-III), tissue factor pathway inhibitor (TFPI), protein C and C1 inhibitor were measured in both rat groups. Results. There was no significant difference in FDP and TAT levels between SHR-SP and WKY rats even at 16-20 weeks when SHR-SP developed severe hypertensive vascular lesions. Contrary to expectations, fluorogenic PT and factor Xa activity were significantly lower in SHR-SP than in WKY rats. While there was no significant difference in AT-III, TFPI and protein C activities between SHR-SP and WKY rats, C1 inhibitor activity was significantly higher in SHR-SP than in WKY rats. The elevated C1 inhibitor activity was inversely correlated with the reduced factor Xa activity. Gel-filtered fractionated plasma with C1 inhibitor activity had an inhibitory effect on the purified rat factor Xa, and immunodepletion of C1 inhibitor from the fractionated plasma attenuated the inhibitory effect. Conclusion. These results suggest that SHR-SP get into a hypocoagulable state rather than a hypercoagulable state, and that the reduction of factor Xa activity in SHR-SP may be related to the elevation of C1 inhibitor activity. (C) Lippincott Williams and Wilkins.

UR - http://www.scopus.com/inward/record.url?scp=0033910299&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033910299&partnerID=8YFLogxK

U2 - 10.1097/00004872-200018070-00012

DO - 10.1097/00004872-200018070-00012

M3 - Article

C2 - 10930188

AN - SCOPUS:0033910299

VL - 18

SP - 901

EP - 909

JO - Journal of Hypertension

JF - Journal of Hypertension

SN - 0263-6352

IS - 7

ER -