Dok2 likely down-regulates Klf1 in mouse erythroleukemia cells

Yuka Tanaka, Kasem Kulkeaw, Tomoko Inoue, Keai Sinn Tan, Yoichi Nakanishi, Senji Shirasawa, Daisuke Sugiyama

Research output: Contribution to journalArticle

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Abstract

Background/Aim: Docking protein 2 (Dok2) is an adapter protein which is involved in hematopoiesis. However, it still remains unclear how Dok2 functions in regulation of transcription of hematopoietic genes. To address this issue, we knocked-down Dok2 mRNA in mouse erythroleukemia cells which highly express Dok2 intrinsically. Materials and Methods: Mouse erythroleukemia cells were transfected with Dok2 siRNA for 24 h and gene expression of erythroid differentiation-related genes, such as GATA binding protein 1 (Gata1), Krüppel-like factor 1 (Klf1), α-globin and β-globin were assessed by real-time polymerase chain reaction. Results: Among the tested genes, expression of Klf1 exhibited a 1.94-fold increase when compared to the control 24 h after transfection. Immunocytochemistry and chromatin immunoprecipitation assays revealed that Dok2 protein localizes in the nucleus and binds to the promoter region of Klf1 gene. Conclusion: Dok2 is able to control Klf1 expression by transcriptional regulation through directly binding to its promoter region.

Original languageEnglish
Pages (from-to)4561-4568
Number of pages8
JournalAnticancer research
Volume34
Issue number8
Publication statusPublished - Aug 1 2014

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Leukemia, Erythroblastic, Acute
Down-Regulation
Proteins
Globins
Genetic Promoter Regions
Genes
Gene Expression
Chromatin Immunoprecipitation
Hematopoiesis
Small Interfering RNA
Transfection
Real-Time Polymerase Chain Reaction
Carrier Proteins
Immunohistochemistry
Messenger RNA

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cite this

Tanaka, Y., Kulkeaw, K., Inoue, T., Tan, K. S., Nakanishi, Y., Shirasawa, S., & Sugiyama, D. (2014). Dok2 likely down-regulates Klf1 in mouse erythroleukemia cells. Anticancer research, 34(8), 4561-4568.

Dok2 likely down-regulates Klf1 in mouse erythroleukemia cells. / Tanaka, Yuka; Kulkeaw, Kasem; Inoue, Tomoko; Tan, Keai Sinn; Nakanishi, Yoichi; Shirasawa, Senji; Sugiyama, Daisuke.

In: Anticancer research, Vol. 34, No. 8, 01.08.2014, p. 4561-4568.

Research output: Contribution to journalArticle

Tanaka, Y, Kulkeaw, K, Inoue, T, Tan, KS, Nakanishi, Y, Shirasawa, S & Sugiyama, D 2014, 'Dok2 likely down-regulates Klf1 in mouse erythroleukemia cells', Anticancer research, vol. 34, no. 8, pp. 4561-4568.
Tanaka Y, Kulkeaw K, Inoue T, Tan KS, Nakanishi Y, Shirasawa S et al. Dok2 likely down-regulates Klf1 in mouse erythroleukemia cells. Anticancer research. 2014 Aug 1;34(8):4561-4568.
Tanaka, Yuka ; Kulkeaw, Kasem ; Inoue, Tomoko ; Tan, Keai Sinn ; Nakanishi, Yoichi ; Shirasawa, Senji ; Sugiyama, Daisuke. / Dok2 likely down-regulates Klf1 in mouse erythroleukemia cells. In: Anticancer research. 2014 ; Vol. 34, No. 8. pp. 4561-4568.
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