Dominant expression of a novel splice variant of caspase-8 in human peripheral blood lymphocytes

Takahiko Horiuchi, Daisuke Himeji, Hiroshi Tsukamoto, Shin Ichi Harashima, Chinami Hashimura, Kenshi Hayashi

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Caspase-8 is an apical and critical proteolytic enzyme in the cascade of apoptosis. As a result of alternative splicing, the generation of at least 7 isoforms of caspase-8 has been reported. The existence of multiple isoforms that lack the essential domains for apoptosis suggests the possible role of these isoforms on the regulation of apoptosis. Here we report a novel longer isoform of caspase-8 (caspase-8L) that was generated by alternative splicing of intron 8, thereby carrying a 136-bp insertion and frame shift of the transcript. The transcript encoded N-terminal two repeats of death effector domain (DED) of caspase-8, but lacking the C-terminal half of the proteolytic domain. Reverse transcriptase (RT)-polymerase chain reaction (PCR) analysis revealed the dominant expression of caspase-8L transcript compared to the intact form of caspase-8 in human peripheral blood lymphocyte (PBL) and T cells. In patients with systemic lupus erythematosus (SLE), imbalanced expression of caspase-8L transcript was identified. These results suggest the important role of caspase-8L in the modulation of apoptosis. (C) 2000 Academic Press.

Original languageEnglish
Pages (from-to)877-881
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume272
Issue number3
DOIs
Publication statusPublished - Jun 16 2000

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Lymphocytes
Caspase 8
Caspases
Protein Isoforms
Blood
Apoptosis
Alternative Splicing
Effector Caspases
Terminal Repeat Sequences
Reverse Transcriptase Polymerase Chain Reaction
Systemic Lupus Erythematosus
T-cells
Introns
Polymerase chain reaction
RNA-Directed DNA Polymerase
Blood Cells
Peptide Hydrolases
T-Lymphocytes
Modulation
human CASP8 protein

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Dominant expression of a novel splice variant of caspase-8 in human peripheral blood lymphocytes. / Horiuchi, Takahiko; Himeji, Daisuke; Tsukamoto, Hiroshi; Harashima, Shin Ichi; Hashimura, Chinami; Hayashi, Kenshi.

In: Biochemical and Biophysical Research Communications, Vol. 272, No. 3, 16.06.2000, p. 877-881.

Research output: Contribution to journalArticle

Horiuchi, Takahiko ; Himeji, Daisuke ; Tsukamoto, Hiroshi ; Harashima, Shin Ichi ; Hashimura, Chinami ; Hayashi, Kenshi. / Dominant expression of a novel splice variant of caspase-8 in human peripheral blood lymphocytes. In: Biochemical and Biophysical Research Communications. 2000 ; Vol. 272, No. 3. pp. 877-881.
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