Dominant expression of interleukin-10 and transforming growth factor-β genes in activated T-cells of chronic active Epstein-Barr virus infection

Shouichi Ohga, Akihiko Nomura, Hidetoshi Takada, Tamami Tanaka, Kenji Furuno, Yasushi Takahata, Naoko Kinukawa, Noriyasu Fukushima, Shosuke Imai, Toshiro Hara

Research output: Contribution to journalArticle

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Abstract

Chronic active Epstein-Barr virus (EBV) infection is a chronic mononucleosis syndrome associated with clonal proliferation of EBV-carrying T-/natural killer (NK)-cells. High levels of circulating EBV and activated T-cells are sustained during the prolonged disease course, whereas it is not clear how ectopic EBV infection in T-/NK-cells has been established and maintained. To assess the biological role of activated T-cells in chronic active EBV infection (CAEBV), EBV DNA and cellular gene expressions in peripheral T-cells were quantified in CAEBV and infectious mononucleosis (IM) patients. In CAEBV, HLA-DR+ T-cells had higher viral load and larger amounts of IFNγ, IL-10, transforming growth factor-beta (TGFβ), and cytotoxic T lymphocyte antigen-4 (CTLA4) mRNA than HLA-DR-T-cells. HLA-DR+ T cells of IM patients transcribed more IFNγ and IL-10 than their HLA-DR-T cells. Expression levels of IFNγ and forkhead box p3 (Foxp3) in CAEBV HLA-DR+ T-cells were higher than in IM HLA-DR+ T-cells. The effective variables to discriminate the positivity of HLA-DR were IL-10, IFNγ, CTLA4, TGFβ, and IL-2 in the order of statistical weight. EBV load in CAEBV T-cells correlated with the expression levels of only IL-10 and TGFβ. These results suggest that CAEBV T-cells are activated to transcribe IFNγ, IL-10, and TGFβ excessively, and the latter two genes are expressed preferentially in the EBV-infected subsets. The dominant expression of regulatory cytokines in T-cells may imply a viral evasion mechanism in the disease.

Original languageEnglish
Pages (from-to)449-458
Number of pages10
JournalJournal of Medical Virology
Volume74
Issue number3
DOIs
Publication statusPublished - Nov 1 2004

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Epstein-Barr Virus Infections
Transforming Growth Factors
Interleukin-10
T-Lymphocytes
HLA-DR Antigens
Genes
Human Herpesvirus 4
Transforming Growth Factor beta
Infectious Mononucleosis
CTLA-4 Antigen
Natural Killer T-Cells
Viral Load
Interleukin-2

All Science Journal Classification (ASJC) codes

  • Virology
  • Infectious Diseases

Cite this

Dominant expression of interleukin-10 and transforming growth factor-β genes in activated T-cells of chronic active Epstein-Barr virus infection. / Ohga, Shouichi; Nomura, Akihiko; Takada, Hidetoshi; Tanaka, Tamami; Furuno, Kenji; Takahata, Yasushi; Kinukawa, Naoko; Fukushima, Noriyasu; Imai, Shosuke; Hara, Toshiro.

In: Journal of Medical Virology, Vol. 74, No. 3, 01.11.2004, p. 449-458.

Research output: Contribution to journalArticle

Ohga, S, Nomura, A, Takada, H, Tanaka, T, Furuno, K, Takahata, Y, Kinukawa, N, Fukushima, N, Imai, S & Hara, T 2004, 'Dominant expression of interleukin-10 and transforming growth factor-β genes in activated T-cells of chronic active Epstein-Barr virus infection', Journal of Medical Virology, vol. 74, no. 3, pp. 449-458. https://doi.org/10.1002/jmv.20197
Ohga, Shouichi ; Nomura, Akihiko ; Takada, Hidetoshi ; Tanaka, Tamami ; Furuno, Kenji ; Takahata, Yasushi ; Kinukawa, Naoko ; Fukushima, Noriyasu ; Imai, Shosuke ; Hara, Toshiro. / Dominant expression of interleukin-10 and transforming growth factor-β genes in activated T-cells of chronic active Epstein-Barr virus infection. In: Journal of Medical Virology. 2004 ; Vol. 74, No. 3. pp. 449-458.
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AU - Takada, Hidetoshi

AU - Tanaka, Tamami

AU - Furuno, Kenji

AU - Takahata, Yasushi

AU - Kinukawa, Naoko

AU - Fukushima, Noriyasu

AU - Imai, Shosuke

AU - Hara, Toshiro

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