Dopaminergic neuronal loss in transgenic mice expressing the Parkinson's disease-associated UCH-L1 I93M mutant

Rieko Setsuie, Yu Lai Wang, Hideki Mochizuki, Hitoshi Osaka, Hideki Hayakawa, Nobutsune Ichihara, Hang Li, Akiko Furuta, Yae Sano, Ying Jie Sun, Jungkee Kwon, Tomohiro Kabuta, Kenji Yoshimi, Shunsuke Aoki, Yoshikuni Mizuno, Mami Noda, Keiji Wada

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Abstract

The I93M mutation in ubiquitin carboxyl-terminal hydrolase L1 (UCH-L1) was reported in one German family with autosomal dominant Parkinson's disease (PD). The causative role of the mutation has, however, been questioned. We generated transgenic (Tg) mice carrying human UCHL1 under control of the PDGF-B promoter; two independent lines were generated with the I93M mutation (a high- and low-expressing line) and one line with wild-type human UCH-L1. We found a significant reduction in the dopaminergic neurons in the substantia nigra and the dopamine content in the striatum in the high-expressing I93M Tg mice as compared with non-Tg mice at 20 weeks of age. Although these changes were absent in the low-expressing I93M Tg mice, 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) treatment profoundly reduced dopaminergic neurons in this line as compared with wild-type Tg or non-Tg mice. Abnormal neuropathologies were also observed, such as silver staining-positive argyrophilic grains in the perikarya of degenerating dopaminergic neurons, in I93M Tg mice. The midbrains of I93M Tg mice contained increased amounts of insoluble UCH-L1 as compared with those of non-Tg mice, perhaps resulting in a toxic gain of function. Collectively, our data represent in vivo evidence that expression of UCHL1I93M leads to the degeneration of dopaminergic neurons.

Original languageEnglish
Pages (from-to)119-129
Number of pages11
JournalNeurochemistry International
Volume50
Issue number1
DOIs
Publication statusPublished - Jan 1 2007

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Hydrolases
Ubiquitin
Transgenic Mice
Dopaminergic Neurons
Parkinson Disease
Mutation
1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine
Silver Staining
Poisons
Substantia Nigra
Mesencephalon
Dopamine

All Science Journal Classification (ASJC) codes

  • Cellular and Molecular Neuroscience
  • Cell Biology

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Dopaminergic neuronal loss in transgenic mice expressing the Parkinson's disease-associated UCH-L1 I93M mutant. / Setsuie, Rieko; Wang, Yu Lai; Mochizuki, Hideki; Osaka, Hitoshi; Hayakawa, Hideki; Ichihara, Nobutsune; Li, Hang; Furuta, Akiko; Sano, Yae; Sun, Ying Jie; Kwon, Jungkee; Kabuta, Tomohiro; Yoshimi, Kenji; Aoki, Shunsuke; Mizuno, Yoshikuni; Noda, Mami; Wada, Keiji.

In: Neurochemistry International, Vol. 50, No. 1, 01.01.2007, p. 119-129.

Research output: Contribution to journalArticle

Setsuie, R, Wang, YL, Mochizuki, H, Osaka, H, Hayakawa, H, Ichihara, N, Li, H, Furuta, A, Sano, Y, Sun, YJ, Kwon, J, Kabuta, T, Yoshimi, K, Aoki, S, Mizuno, Y, Noda, M & Wada, K 2007, 'Dopaminergic neuronal loss in transgenic mice expressing the Parkinson's disease-associated UCH-L1 I93M mutant', Neurochemistry International, vol. 50, no. 1, pp. 119-129. https://doi.org/10.1016/j.neuint.2006.07.015
Setsuie, Rieko ; Wang, Yu Lai ; Mochizuki, Hideki ; Osaka, Hitoshi ; Hayakawa, Hideki ; Ichihara, Nobutsune ; Li, Hang ; Furuta, Akiko ; Sano, Yae ; Sun, Ying Jie ; Kwon, Jungkee ; Kabuta, Tomohiro ; Yoshimi, Kenji ; Aoki, Shunsuke ; Mizuno, Yoshikuni ; Noda, Mami ; Wada, Keiji. / Dopaminergic neuronal loss in transgenic mice expressing the Parkinson's disease-associated UCH-L1 I93M mutant. In: Neurochemistry International. 2007 ; Vol. 50, No. 1. pp. 119-129.
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