Down-regulation of human telomeric protein TRF1 gene expression during myeloid differentiation in human hematopoietic cells.

T. Otsuka, N. Uchida, F. Arima, H. Shigematsu, T. Fukuyama, M. Maeda, Y. Sugio, Y. Itoh, Y. Niho

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

The maintenance of telomere length is crucial for cell survival. Recently, it has been indicated that the human telomeric protein TRF1 is involved in the negative feedback mechanism that stabilizes telomere length. We studied TRF1 mRNA expression in hematopoietic cells to clarify the relation between TRF1 and telomerase by semiquantitative reverse transcriptase-polymerase chain reaction. In polymorphonuclear cells and monocytes isolated from peripheral blood, relatively low levels of TRF1 mRNA expression were seen, compared with those of lymphocytes and CD34+. We then assessed TRF1 mRNA expression in CD34+ cells cultured in vitro with growth factors. After 4 weeks of culture, all the cells showed myeloid differentiation, and telomerase activity was down-regulated. TRF1 mRNA was expressed in CD34+ cells but was down-regulated in cells cultured for 4 weeks. We conclude that TRF1 mRNA expression is down-regulated in accordance with telomerase down-regulation during the course of myeloid differentiation.

Original languageEnglish
Pages (from-to)334-339
Number of pages6
JournalInternational journal of hematology
Volume71
Issue number4
Publication statusPublished - Jun 2000

All Science Journal Classification (ASJC) codes

  • Hematology

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