Down-Regulation of microRNA-132 is Associated with Poor Prognosis of Colorectal Cancer

Yukako Mokutani, Mamoru Uemura, Koji Munakata, Daisuke Okuzaki, Naotsugu Haraguchi, Hidekazu Takahashi, Junichi Nishimura, Taishi Hata, Kohei Murata, Ichiro Takemasa, Tsunekazu Mizushima, Yuichiro Doki, Masaki Mori, Hirofumi Yamamoto

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Background: Given the role of microRNA in colorectal cancer (CRC) progression, we explored the association between microRNA (miRNA) expression and CRC-related prognosis. Methods: Three types of tissue samples (primary CRC lesions without liver metastasis, primary lesions with liver metastasis, and liver metastatic tissues) were used for miRNA profiling to identify differentially expressed miRNA. Quantitative real-time PCR was used to examine miRNA expression in CRC cells and in tumor tissues. Results: MiR-132 was significantly down-regulated in primary CRC tissues with liver metastasis and liver metastatic lesions compared to primary lesions without liver metastasis. Multivariate analysis for overall survival indicated that low miR-132 expression was an independent prognostic factor for CRC patients (overall survival P = 0.040, disease-free survival P = 0.015). Ectopic expression of miR-132 significantly inhibited cell proliferation and cell invasion. The luciferase reporter assay revealed that anoctamin 1 (ANO1) was a direct target of miR-132. Kaplan–Meier survival curves showed that high ANO1 expression was a significant prognostic factor for overall survival of patients with CRC (P = 0.0344). Conclusions: Down-regulation of miR-132 is associated with poor prognosis in CRC. ANO1 could be one of the crucial targets of miR-132 in CRC.

Original languageEnglish
Pages (from-to)599-608
Number of pages10
JournalAnnals of Surgical Oncology
Volume23
DOIs
Publication statusPublished - Dec 1 2016

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MicroRNAs
Colorectal Neoplasms
Down-Regulation
Liver
Neoplasm Metastasis
Survival
Luciferases
Disease-Free Survival
Real-Time Polymerase Chain Reaction
Multivariate Analysis
Cell Proliferation

All Science Journal Classification (ASJC) codes

  • Surgery
  • Oncology

Cite this

Mokutani, Y., Uemura, M., Munakata, K., Okuzaki, D., Haraguchi, N., Takahashi, H., ... Yamamoto, H. (2016). Down-Regulation of microRNA-132 is Associated with Poor Prognosis of Colorectal Cancer. Annals of Surgical Oncology, 23, 599-608. https://doi.org/10.1245/s10434-016-5133-3

Down-Regulation of microRNA-132 is Associated with Poor Prognosis of Colorectal Cancer. / Mokutani, Yukako; Uemura, Mamoru; Munakata, Koji; Okuzaki, Daisuke; Haraguchi, Naotsugu; Takahashi, Hidekazu; Nishimura, Junichi; Hata, Taishi; Murata, Kohei; Takemasa, Ichiro; Mizushima, Tsunekazu; Doki, Yuichiro; Mori, Masaki; Yamamoto, Hirofumi.

In: Annals of Surgical Oncology, Vol. 23, 01.12.2016, p. 599-608.

Research output: Contribution to journalArticle

Mokutani, Y, Uemura, M, Munakata, K, Okuzaki, D, Haraguchi, N, Takahashi, H, Nishimura, J, Hata, T, Murata, K, Takemasa, I, Mizushima, T, Doki, Y, Mori, M & Yamamoto, H 2016, 'Down-Regulation of microRNA-132 is Associated with Poor Prognosis of Colorectal Cancer', Annals of Surgical Oncology, vol. 23, pp. 599-608. https://doi.org/10.1245/s10434-016-5133-3
Mokutani Y, Uemura M, Munakata K, Okuzaki D, Haraguchi N, Takahashi H et al. Down-Regulation of microRNA-132 is Associated with Poor Prognosis of Colorectal Cancer. Annals of Surgical Oncology. 2016 Dec 1;23:599-608. https://doi.org/10.1245/s10434-016-5133-3
Mokutani, Yukako ; Uemura, Mamoru ; Munakata, Koji ; Okuzaki, Daisuke ; Haraguchi, Naotsugu ; Takahashi, Hidekazu ; Nishimura, Junichi ; Hata, Taishi ; Murata, Kohei ; Takemasa, Ichiro ; Mizushima, Tsunekazu ; Doki, Yuichiro ; Mori, Masaki ; Yamamoto, Hirofumi. / Down-Regulation of microRNA-132 is Associated with Poor Prognosis of Colorectal Cancer. In: Annals of Surgical Oncology. 2016 ; Vol. 23. pp. 599-608.
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abstract = "Background: Given the role of microRNA in colorectal cancer (CRC) progression, we explored the association between microRNA (miRNA) expression and CRC-related prognosis. Methods: Three types of tissue samples (primary CRC lesions without liver metastasis, primary lesions with liver metastasis, and liver metastatic tissues) were used for miRNA profiling to identify differentially expressed miRNA. Quantitative real-time PCR was used to examine miRNA expression in CRC cells and in tumor tissues. Results: MiR-132 was significantly down-regulated in primary CRC tissues with liver metastasis and liver metastatic lesions compared to primary lesions without liver metastasis. Multivariate analysis for overall survival indicated that low miR-132 expression was an independent prognostic factor for CRC patients (overall survival P = 0.040, disease-free survival P = 0.015). Ectopic expression of miR-132 significantly inhibited cell proliferation and cell invasion. The luciferase reporter assay revealed that anoctamin 1 (ANO1) was a direct target of miR-132. Kaplan–Meier survival curves showed that high ANO1 expression was a significant prognostic factor for overall survival of patients with CRC (P = 0.0344). Conclusions: Down-regulation of miR-132 is associated with poor prognosis in CRC. ANO1 could be one of the crucial targets of miR-132 in CRC.",
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AU - Okuzaki, Daisuke

AU - Haraguchi, Naotsugu

AU - Takahashi, Hidekazu

AU - Nishimura, Junichi

AU - Hata, Taishi

AU - Murata, Kohei

AU - Takemasa, Ichiro

AU - Mizushima, Tsunekazu

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