Deregulation of survivin expression is implicated in tumorigenesis. To examine the regulation of survivin expression in response to DNA damage, we exposed A549 human lung cancer cells to ultraviolet C (UVC) radiation, which induces DNA single-strand breakage. UVC irradiation induced G2-M arrest that was accompanied by accumulation of p53 and subsequent down-regulation of survivin. Depletion of p53 by RNA interference prevented the UVC-induced down-regulation of survivin. Furthermore, depletion of survivin resulted in G2-M arrest, suggesting that down-regulation of survivin by p53 contributes to the p53-dependent G2-M checkpoint triggered by DNA damage.
All Science Journal Classification (ASJC) codes
- Cancer Research