Downregulation of P2X3 receptor-dependent sensory functions in A/J inbred mouse strain

Makoto Tsuda, Yukari Shigemoto-Mogami, Shinya Ueno, Schuichi Koizumi, Hiroshi Ueda, Toshihiko Iwanaga, Kazuhide Inoue

Research output: Contribution to journalArticle

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Abstract

There is large variability in the various pain responses including those to tissue injury among inbred mouse strains. However, the determinant factors for the strain-specific differences remain unknown. The P2X3 sensory-specific ATP-gated channel has been implicated as a damage-sensing molecule that evokes a pain sensation by receiving endogenous ATP from injured tissue. In this study, to clarify the contribution of the sensory P2X3 signalling to strain-specific differences in tissue injury pain, we examined whether the P2X3-mediated in vivo and in vitro responses in dorsal root ganglion (DRG) neurons are changed in the A/J inbred mouse strain, which is known to be resistant to tissue injury pain caused by formalin. Here we found that A/J mice exhibited a low magnitude of nocifensive behaviour induced by the P2X agonist α,β-methylene ATP (αβmeATP) into the hindpaw compared with C57BL/6 J mice. This behaviour was blocked by P2X3 antisense oligodeoxynucleotides. The low magnitude of the in vivo pain sensation could be observed similarly in the in vitro response; the increase in the intracellular Ca2+ increase by αβmeATP in capsaicin-sensitive DRG neurons from A/J mice was significantly lower than that from C57BL/6 J mice. In A/J DRG neurons the P2X3 protein level was significantly lower compared with C57BL/6 J DRG neurons. The change in P2X3 protein was selective because P2X2 protein was expressed equally in both strains. The present study suggests that the downregulation of sensory P2X3 could be one of the molecular predispositions to low sensitivity to tissue injury pain in the A/J inbred mouse strain.

Original languageEnglish
Pages (from-to)1444-1450
Number of pages7
JournalEuropean Journal of Neuroscience
Volume15
Issue number9
DOIs
Publication statusPublished - Jan 1 2002

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Purinergic P2X3 Receptors
Inbred Strains Mice
Spinal Ganglia
Nociceptive Pain
Down-Regulation
Adenosine Triphosphate
Neurons
Wounds and Injuries
Pain
Proteins
Oligodeoxyribonucleotides
Capsaicin
Formaldehyde

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

Downregulation of P2X3 receptor-dependent sensory functions in A/J inbred mouse strain. / Tsuda, Makoto; Shigemoto-Mogami, Yukari; Ueno, Shinya; Koizumi, Schuichi; Ueda, Hiroshi; Iwanaga, Toshihiko; Inoue, Kazuhide.

In: European Journal of Neuroscience, Vol. 15, No. 9, 01.01.2002, p. 1444-1450.

Research output: Contribution to journalArticle

Tsuda, Makoto ; Shigemoto-Mogami, Yukari ; Ueno, Shinya ; Koizumi, Schuichi ; Ueda, Hiroshi ; Iwanaga, Toshihiko ; Inoue, Kazuhide. / Downregulation of P2X3 receptor-dependent sensory functions in A/J inbred mouse strain. In: European Journal of Neuroscience. 2002 ; Vol. 15, No. 9. pp. 1444-1450.
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