Abstract
There is large variability in the various pain responses including those to tissue injury among inbred mouse strains. However, the determinant factors for the strain-specific differences remain unknown. The P2X3 sensory-specific ATP-gated channel has been implicated as a damage-sensing molecule that evokes a pain sensation by receiving endogenous ATP from injured tissue. In this study, to clarify the contribution of the sensory P2X3 signalling to strain-specific differences in tissue injury pain, we examined whether the P2X3-mediated in vivo and in vitro responses in dorsal root ganglion (DRG) neurons are changed in the A/J inbred mouse strain, which is known to be resistant to tissue injury pain caused by formalin. Here we found that A/J mice exhibited a low magnitude of nocifensive behaviour induced by the P2X agonist α,β-methylene ATP (αβmeATP) into the hindpaw compared with C57BL/6 J mice. This behaviour was blocked by P2X3 antisense oligodeoxynucleotides. The low magnitude of the in vivo pain sensation could be observed similarly in the in vitro response; the increase in the intracellular Ca2+ increase by αβmeATP in capsaicin-sensitive DRG neurons from A/J mice was significantly lower than that from C57BL/6 J mice. In A/J DRG neurons the P2X3 protein level was significantly lower compared with C57BL/6 J DRG neurons. The change in P2X3 protein was selective because P2X2 protein was expressed equally in both strains. The present study suggests that the downregulation of sensory P2X3 could be one of the molecular predispositions to low sensitivity to tissue injury pain in the A/J inbred mouse strain.
| Original language | English |
|---|---|
| Pages (from-to) | 1444-1450 |
| Number of pages | 7 |
| Journal | European Journal of Neuroscience |
| Volume | 15 |
| Issue number | 9 |
| DOIs | |
| Publication status | Published - Jan 1 2002 |
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All Science Journal Classification (ASJC) codes
- Neuroscience(all)
Cite this
Downregulation of P2X3 receptor-dependent sensory functions in A/J inbred mouse strain. / Tsuda, Makoto; Shigemoto-Mogami, Yukari; Ueno, Shinya; Koizumi, Schuichi; Ueda, Hiroshi; Iwanaga, Toshihiko; Inoue, Kazuhide.
In: European Journal of Neuroscience, Vol. 15, No. 9, 01.01.2002, p. 1444-1450.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Downregulation of P2X3 receptor-dependent sensory functions in A/J inbred mouse strain
AU - Tsuda, Makoto
AU - Shigemoto-Mogami, Yukari
AU - Ueno, Shinya
AU - Koizumi, Schuichi
AU - Ueda, Hiroshi
AU - Iwanaga, Toshihiko
AU - Inoue, Kazuhide
PY - 2002/1/1
Y1 - 2002/1/1
N2 - There is large variability in the various pain responses including those to tissue injury among inbred mouse strains. However, the determinant factors for the strain-specific differences remain unknown. The P2X3 sensory-specific ATP-gated channel has been implicated as a damage-sensing molecule that evokes a pain sensation by receiving endogenous ATP from injured tissue. In this study, to clarify the contribution of the sensory P2X3 signalling to strain-specific differences in tissue injury pain, we examined whether the P2X3-mediated in vivo and in vitro responses in dorsal root ganglion (DRG) neurons are changed in the A/J inbred mouse strain, which is known to be resistant to tissue injury pain caused by formalin. Here we found that A/J mice exhibited a low magnitude of nocifensive behaviour induced by the P2X agonist α,β-methylene ATP (αβmeATP) into the hindpaw compared with C57BL/6 J mice. This behaviour was blocked by P2X3 antisense oligodeoxynucleotides. The low magnitude of the in vivo pain sensation could be observed similarly in the in vitro response; the increase in the intracellular Ca2+ increase by αβmeATP in capsaicin-sensitive DRG neurons from A/J mice was significantly lower than that from C57BL/6 J mice. In A/J DRG neurons the P2X3 protein level was significantly lower compared with C57BL/6 J DRG neurons. The change in P2X3 protein was selective because P2X2 protein was expressed equally in both strains. The present study suggests that the downregulation of sensory P2X3 could be one of the molecular predispositions to low sensitivity to tissue injury pain in the A/J inbred mouse strain.
AB - There is large variability in the various pain responses including those to tissue injury among inbred mouse strains. However, the determinant factors for the strain-specific differences remain unknown. The P2X3 sensory-specific ATP-gated channel has been implicated as a damage-sensing molecule that evokes a pain sensation by receiving endogenous ATP from injured tissue. In this study, to clarify the contribution of the sensory P2X3 signalling to strain-specific differences in tissue injury pain, we examined whether the P2X3-mediated in vivo and in vitro responses in dorsal root ganglion (DRG) neurons are changed in the A/J inbred mouse strain, which is known to be resistant to tissue injury pain caused by formalin. Here we found that A/J mice exhibited a low magnitude of nocifensive behaviour induced by the P2X agonist α,β-methylene ATP (αβmeATP) into the hindpaw compared with C57BL/6 J mice. This behaviour was blocked by P2X3 antisense oligodeoxynucleotides. The low magnitude of the in vivo pain sensation could be observed similarly in the in vitro response; the increase in the intracellular Ca2+ increase by αβmeATP in capsaicin-sensitive DRG neurons from A/J mice was significantly lower than that from C57BL/6 J mice. In A/J DRG neurons the P2X3 protein level was significantly lower compared with C57BL/6 J DRG neurons. The change in P2X3 protein was selective because P2X2 protein was expressed equally in both strains. The present study suggests that the downregulation of sensory P2X3 could be one of the molecular predispositions to low sensitivity to tissue injury pain in the A/J inbred mouse strain.
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UR - http://www.scopus.com/inward/citedby.url?scp=0036025431&partnerID=8YFLogxK
U2 - 10.1046/j.1460-9568.2002.01982.x
DO - 10.1046/j.1460-9568.2002.01982.x
M3 - Article
C2 - 12028354
AN - SCOPUS:0036025431
VL - 15
SP - 1444
EP - 1450
JO - European Journal of Neuroscience
JF - European Journal of Neuroscience
SN - 0953-816X
IS - 9
ER -