Downregulation of prolactin-releasing peptide gene expression in the hypothalamus and brainstem of diabetic rats

Takashi Mera, Hiroaki Fujihara, Jun Saito, Makoto Kawasaki, Hirofumi Hashimoto, Takeshi Saito, Minori Shibata, Tatsushi Onaka, Yoshiya Tanaka, Takakazu Oka, Sadatoshi Tsuji, Yoichi Ueta

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

We investigated the prolactin-releasing peptide (PrRP) mRNA levels in the hypothalamus and brainstem of streptozotocin (STZ)-induced diabetic rats and fa/fa Zucker diabetic rats, using in situ hybridization histochemistry. PrRP mRNA levels in the hypothalamus and brainstem of STZ-induced diabetic rats were significantly reduced in comparison with those of control rats. PrRP mRNA levels in the diabetic rats were reversed by both insulin and leptin. PrRP mRNA levels in the fa/fa diabetic rats were significantly reduced in comparison with those of Fa/? rats. PrRP mRNA levels in the fa/fa diabetic rats were significantly increased by insulin-treatment, but did not reach control levels in the Fa/? rats. We also investigated the effect of restraint stress on PrRP mRNA levels in STZ-induced diabetic rats. The PrRP mRNA levels in the control and the STZ-induced diabetic rats increased significantly after restraint stress. The diabetic condition and insulin-treatment may affect the regulation of PrRP gene expression via leptin and other factors, such as plasma glucose level. The diabetic condition may not impair the role of PrRP as a stress mediator.

Original languageEnglish
Pages (from-to)1596-1604
Number of pages9
JournalPeptides
Volume28
Issue number8
DOIs
Publication statusPublished - Aug 2007

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Physiology
  • Endocrinology
  • Cellular and Molecular Neuroscience

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