Downregulation of ST6GALNAC1 is associated with esophageal squamous cell carcinoma development

Takeshi Iwaya, Genta Sawada, Suburu Amano, Kohei Kume, Chie Ito, Fumitaka Endo, Masafumi Konosu, Yoshihiro Shioi, Yuji Akiyama, Takeshi Takahara, Koki Otsuka, Hiroyuki Nitta, Keisuke Koeda, Masaru Mizuno, Satoshi Nishizuka, Akira Sasaki, Koshi Mimori

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Tylosis is an inherited disorder characterized by abnormal palmoplantar skin thickening and a highly elevated risk of esophageal squamous cell carcinoma (ESCC). Analyses of tylosis in families have localized the responsible gene locus to a region of chromosome 17q25.1. Frequent loss of heterozygosity (LOH) in 17q25.1 was also observed in the sporadic form of ESCC. A putative tumor suppressor gene for ESCC may exist at this locus. We investigated the expression patterns of genes on 17q25.1 in tumor and corresponding normal tissues from patients with sporadic ESCC using RNA sequence analysis. For candidate genes, quantitative real-time reverse transcription-PCR (qRT-PCR), direct sequence, LOH and methylation analyses were performed using 93 clinical ESCC samples and 10 cell lines. A significant downregulation of ST6GALNAC1 was demonstrated in ESCC tissues compared to its expression in normal tissues by qRT-PCR (n=93, p<0.0001). Frequent LOH (17/27, 62.9%) and hyper-methylation in ST6GALNAC1 were also observed in all cell lines. Our results indicated that ST6GALNAC1 was downregulated in sporadic ESCC via hyper-methylation and LOH, and it may be a candidate responsible gene for ESCC. Furthermore, recent studies suggest that multiple genes on chromosome 17q25 are involved in ESCC development.

Original languageEnglish
Pages (from-to)441-447
Number of pages7
JournalInternational journal of oncology
Volume50
Issue number2
DOIs
Publication statusPublished - Feb 2017

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Down-Regulation
Loss of Heterozygosity
Keratoderma, Palmoplantar, Diffuse
Methylation
Genes
Reverse Transcription
Chromosomes
RNA Sequence Analysis
Esophageal Squamous Cell Carcinoma
Cell Line
Polymerase Chain Reaction
Tumor Suppressor Genes
Gene Expression
Skin
Neoplasms

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cite this

Downregulation of ST6GALNAC1 is associated with esophageal squamous cell carcinoma development. / Iwaya, Takeshi; Sawada, Genta; Amano, Suburu; Kume, Kohei; Ito, Chie; Endo, Fumitaka; Konosu, Masafumi; Shioi, Yoshihiro; Akiyama, Yuji; Takahara, Takeshi; Otsuka, Koki; Nitta, Hiroyuki; Koeda, Keisuke; Mizuno, Masaru; Nishizuka, Satoshi; Sasaki, Akira; Mimori, Koshi.

In: International journal of oncology, Vol. 50, No. 2, 02.2017, p. 441-447.

Research output: Contribution to journalArticle

Iwaya, T, Sawada, G, Amano, S, Kume, K, Ito, C, Endo, F, Konosu, M, Shioi, Y, Akiyama, Y, Takahara, T, Otsuka, K, Nitta, H, Koeda, K, Mizuno, M, Nishizuka, S, Sasaki, A & Mimori, K 2017, 'Downregulation of ST6GALNAC1 is associated with esophageal squamous cell carcinoma development', International journal of oncology, vol. 50, no. 2, pp. 441-447. https://doi.org/10.3892/ijo.2016.3817
Iwaya, Takeshi ; Sawada, Genta ; Amano, Suburu ; Kume, Kohei ; Ito, Chie ; Endo, Fumitaka ; Konosu, Masafumi ; Shioi, Yoshihiro ; Akiyama, Yuji ; Takahara, Takeshi ; Otsuka, Koki ; Nitta, Hiroyuki ; Koeda, Keisuke ; Mizuno, Masaru ; Nishizuka, Satoshi ; Sasaki, Akira ; Mimori, Koshi. / Downregulation of ST6GALNAC1 is associated with esophageal squamous cell carcinoma development. In: International journal of oncology. 2017 ; Vol. 50, No. 2. pp. 441-447.
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AU - Sawada, Genta

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AU - Kume, Kohei

AU - Ito, Chie

AU - Endo, Fumitaka

AU - Konosu, Masafumi

AU - Shioi, Yoshihiro

AU - Akiyama, Yuji

AU - Takahara, Takeshi

AU - Otsuka, Koki

AU - Nitta, Hiroyuki

AU - Koeda, Keisuke

AU - Mizuno, Masaru

AU - Nishizuka, Satoshi

AU - Sasaki, Akira

AU - Mimori, Koshi

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