TY - JOUR
T1 - Droplet-based microfluidic sensing system for rapid fish freshness determination
AU - Itoh, Daisuke
AU - Sassa, Fumihiro
AU - Nishi, Taiji
AU - Kani, Yoko
AU - Murata, Masakazu
AU - Suzuki, Hiroaki
N1 - Funding Information:
This study was supported by the Research and Development Projects for Application in Promoting New Policy of Agriculture Forestry and Fisheries by the Ministry of Agriculture, Forestry and Fisheries, Japan .
Copyright:
Copyright 2012 Elsevier B.V., All rights reserved.
PY - 2012/8
Y1 - 2012/8
N2 - A microfluidic device was constructed for on-site determination of fish freshness by using adenosine-5′-triphosphate (ATP) concentration as an indicator of freshness. In the device, extracts from fish and reagent solutions were processed in the form of droplets separated by air. The volumes of the droplets of the extract solution, a solution containing enzyme substrates, and a washing buffer solution were measured in branched flow channels. To simplify the structure and procedure for mixing solutions, a wider portion was formed in one flow channel to merge the droplets. A row of droplets was ejected from each of three branched flow channels, merged in the wider portion, and transported to a sensing region located downstream. ATP was detected using two enzymatic reactions involving glycerol kinase (GK) and glycerol-3-phosphate oxidase (G3PO). A linear relationship between the generated current and ATP concentration was confirmed, and ATP detection from jack mackerel extracts was carried out. A distinct change in the current was observed with the fresh extracts as in the case of standard solutions. The obtained data correlated well with those obtained by high-performance liquid chromatography (HPLC).
AB - A microfluidic device was constructed for on-site determination of fish freshness by using adenosine-5′-triphosphate (ATP) concentration as an indicator of freshness. In the device, extracts from fish and reagent solutions were processed in the form of droplets separated by air. The volumes of the droplets of the extract solution, a solution containing enzyme substrates, and a washing buffer solution were measured in branched flow channels. To simplify the structure and procedure for mixing solutions, a wider portion was formed in one flow channel to merge the droplets. A row of droplets was ejected from each of three branched flow channels, merged in the wider portion, and transported to a sensing region located downstream. ATP was detected using two enzymatic reactions involving glycerol kinase (GK) and glycerol-3-phosphate oxidase (G3PO). A linear relationship between the generated current and ATP concentration was confirmed, and ATP detection from jack mackerel extracts was carried out. A distinct change in the current was observed with the fresh extracts as in the case of standard solutions. The obtained data correlated well with those obtained by high-performance liquid chromatography (HPLC).
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U2 - 10.1016/j.snb.2012.05.043
DO - 10.1016/j.snb.2012.05.043
M3 - Article
AN - SCOPUS:84864282347
SN - 0925-4005
VL - 171-172
SP - 619
EP - 626
JO - Sensors and Actuators B: Chemical
JF - Sensors and Actuators B: Chemical
ER -