Drosophila actin mutants and the study of myofibrillar assembly and function.

J. C. Sparrow, Douglas Robert Drummond, E. S. Hennessey, J. D. Clayton, F. B. Lindegaard

Research output: Contribution to journalReview article

10 Citations (Scopus)

Abstract

The use of Drosophila mutations in the indirect flight muscle-specific actin gene, Act88F, to study actin structure/function and its assembly into thin filaments during myofibrillogenesis is described. Mutants with different phenotypic effects are discussed and attempts made to correlate the different properties of the mutants in vivo-myofibrillar structure, actin synthesis, accumulation and stability, heat shock response induction-with properties of the same mutations expressed by in vitro transcription/translation of the cloned actin genes-co-polymerisation, thermostability and protein conformation. Few of the properties show a complete correlation between the different classes of mutants. The nature of the diversity of the mutant effects is discussed. Questions as to how this will help in elucidating the molecular effects of the mutations and the assembly of thin filaments and myofibrils are considered. In addition, the efficacy of the co-polymerisation assay is examined. The post-translational processing of this actin-by N-terminal processing, methylation and ubiquitination-are described. Data is presented that inhibition of the N-terminal processing of actin in vitro affects the ability of the actin to copolymerise, and makes unprocessed actin behave as a capping protein. The possible in vivo importance of this phenomenon is discussed.

Original languageEnglish
Pages (from-to)111-129
Number of pages19
JournalSymposia of the Society for Experimental Biology
Volume46
Publication statusPublished - Jan 1 1992
Externally publishedYes

Fingerprint

Drosophila
Actins
Polymerization
Mutation
Heat-Shock Response
Protein Conformation
Muscle Development
Myofibrils
Ubiquitination
Methylation
Genes
Muscles
Proteins

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

Drosophila actin mutants and the study of myofibrillar assembly and function. / Sparrow, J. C.; Drummond, Douglas Robert; Hennessey, E. S.; Clayton, J. D.; Lindegaard, F. B.

In: Symposia of the Society for Experimental Biology, Vol. 46, 01.01.1992, p. 111-129.

Research output: Contribution to journalReview article

Sparrow, J. C. ; Drummond, Douglas Robert ; Hennessey, E. S. ; Clayton, J. D. ; Lindegaard, F. B. / Drosophila actin mutants and the study of myofibrillar assembly and function. In: Symposia of the Society for Experimental Biology. 1992 ; Vol. 46. pp. 111-129.
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