Drosophila cryb mutation reveals two circadian clocks that drive locomotor rhythm and have different responsiveness to light

Taishi Yoshii, Yuriko Funada, Tadashi Ibuki-Ishibashi, Akira Matsumoto, Teiichi Tanimura, Kenji Tomioka

Research output: Contribution to journalArticle

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Abstract

Cryptochrome (CRY) is a blue-light-absorbing protein involved in the photic entrainment of the circadian clock in Drosophila melanogaster. We have investigated the locomotor activity rhythms of flies carrying cryb mutant and revealed that they have two separate circadian oscillators with different responsiveness to light. When kept in constant light conditions, wild-type flies became arrhythmic, while cryb mutant flies exhibited free-running rhythms with two rhythmic components, one with a shorter and the other with a longer free-running period. The rhythm dissociation was dependent on the light intensities: the higher the light intensities, the greater the proportion of animals exhibiting the two oscillations. External photoreceptors including the compound eyes and the ocelli are the likely photoreceptors for the rhythm dissociation, since rhythm dissociation was prevented in so 1;cryb and norpAP41;cryb double mutant flies. Immunohistochemical analysis demonstrated that the PERIOD expression rhythms in ventrally located lateral neurons (LNvs) occurred synchronously with the shorter period component, while those in the dorsally located per-expressing neurons showed PER expression most likely related to the longer period component, in addition to that synchronized to the LNvs. These results suggest that the Drosophila locomotor rhythms are driven by two separate per-dependent clocks, responding differentially to constant light.

Original languageEnglish
Pages (from-to)479-488
Number of pages10
JournalJournal of insect physiology
Volume50
Issue number6
DOIs
Publication statusPublished - Jun 1 2004

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Circadian Clocks
circadian rhythm
Drosophila
mutation
Light
Diptera
Mutation
photoreceptors
mutants
light intensity
neurons
ocelli
compound eyes
blue light
Cryptochromes
locomotion
Drosophila melanogaster
Neurons
oscillation
Locomotion

All Science Journal Classification (ASJC) codes

  • Physiology
  • Insect Science

Cite this

Drosophila cryb mutation reveals two circadian clocks that drive locomotor rhythm and have different responsiveness to light. / Yoshii, Taishi; Funada, Yuriko; Ibuki-Ishibashi, Tadashi; Matsumoto, Akira; Tanimura, Teiichi; Tomioka, Kenji.

In: Journal of insect physiology, Vol. 50, No. 6, 01.06.2004, p. 479-488.

Research output: Contribution to journalArticle

Yoshii, Taishi ; Funada, Yuriko ; Ibuki-Ishibashi, Tadashi ; Matsumoto, Akira ; Tanimura, Teiichi ; Tomioka, Kenji. / Drosophila cryb mutation reveals two circadian clocks that drive locomotor rhythm and have different responsiveness to light. In: Journal of insect physiology. 2004 ; Vol. 50, No. 6. pp. 479-488.
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