Drosophila mitochondrial transcription factor B1 modulates mitochondrial translation but not transcription or DNA copy number in Schneider cells

Yuichi Matsushima, Cristina Adán, Rafael Garesse, Laurie S. Kaguni

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

We report the cloning and molecular analysis of Drosophila mitochondrial transcription factor (d-mtTF) B1. An RNA interference (RNAi) construct was designed that reduces expression of d-mtTFB1 to 5% of its normal level in Schneider cells. In striking contrast with our previous study on d-mtTFB2, we found that RNAi knockdown of d-mtTFB1 does not change the abundance of specific mitochondrial RNA transcripts, nor does it affect the copy number of mitochondrial DNA. In a corollary manner, overespression of d-mtTFB1 did not increase either the abundance of mitochondrial RNA transcripts or mitochondrial DNA copy number. Our data suggest that, unlike d-mtTFB2, d-mtTFB1 does not have a critical role in either transcription or regulation of the copy number of mitochondrial DNA. Instead, because we found that RNAi knockdown of d-mtTFB1 reduces mitochondrial protein synthesis, we propose that it serves its primary role in modulating translation. Our work represents the first study to document the role of mtTFB1 in vivo and establishes clearly functional differences between mtTFB1 and mtTFB2.

Original languageEnglish
Pages (from-to)16815-16820
Number of pages6
JournalJournal of Biological Chemistry
Volume280
Issue number17
DOIs
Publication statusPublished - Apr 29 2005
Externally publishedYes

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Transcription
RNA Interference
Mitochondrial DNA
Drosophila
RNA
DNA
Cloning
Mitochondrial Proteins
Molecular Cloning
human TFB1M protein
mitochondrial RNA

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Drosophila mitochondrial transcription factor B1 modulates mitochondrial translation but not transcription or DNA copy number in Schneider cells. / Matsushima, Yuichi; Adán, Cristina; Garesse, Rafael; Kaguni, Laurie S.

In: Journal of Biological Chemistry, Vol. 280, No. 17, 29.04.2005, p. 16815-16820.

Research output: Contribution to journalArticle

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