TY - JOUR
T1 - Dual crosslinked hydrogel nanoparticles by nanogel bottom-up method for sustained-release delivery
AU - Shimoda, Asako
AU - Sawada, Shin ichi
AU - Kano, Arihiro
AU - Maruyama, Atsushi
AU - Moquin, Alexandre
AU - Winnik, Françoise M.
AU - Akiyoshi, Kazunari
N1 - Funding Information:
This work was supported by a Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology (No. 20240047 and 22114009 ). This work was performed under the Cooperative Research Program of Network Joint Research Center for Materials and Devices (Institute for Materials Chemistry and Engineering, Kyushu University).
PY - 2012/11/1
Y1 - 2012/11/1
N2 - Polysaccharide-PEG hybrid nanogels (CHPOA-PEGSH) crosslinked by both covalent ester bonds and physical interactions were prepared by the reaction of a thiol-modified poly(ethylene glycol) (PEGSH) with acryloyl-modified cholesterol-bearing pullulan (CHPOA). Experimental parameters, including CHPOA concentration, the degree of acryloyl substitution of CHPOA, and the initial amounts of CHPOA and PEGSH, were modified in order to assess their effect on the size of the nanogels (50-150nm) and on their degradation kinetics, monitored by dynamic light scattering (DLS) and asymmetrical flow field-flow fractionation (AF4) chromatography. Rhodamine-labeled nanogels were injected intravenously into mice and their concentration in blood was determined by a fluorescence assay as a function of post-injection time. The elimination half-life (t 1/2) of CHPOA-PEGSH nanoparticles was about 15-fold longer (18h) than that of CHP nanogels (1.2h). The half-life enhancement of CHPOA-PEGSH was attributed to the presence of the crosslinker PEG chains, which prevent non-specific protein adsorption, and to the slow hydrolysis kinetics of the crosslinking esters in the biological milieu. The hybrid CHPOA-PEGSH nanogels are expected to be useful as injectable nanocarriers for drugs and proteins, in view of their low surface fouling and slow hydrolysis rate.
AB - Polysaccharide-PEG hybrid nanogels (CHPOA-PEGSH) crosslinked by both covalent ester bonds and physical interactions were prepared by the reaction of a thiol-modified poly(ethylene glycol) (PEGSH) with acryloyl-modified cholesterol-bearing pullulan (CHPOA). Experimental parameters, including CHPOA concentration, the degree of acryloyl substitution of CHPOA, and the initial amounts of CHPOA and PEGSH, were modified in order to assess their effect on the size of the nanogels (50-150nm) and on their degradation kinetics, monitored by dynamic light scattering (DLS) and asymmetrical flow field-flow fractionation (AF4) chromatography. Rhodamine-labeled nanogels were injected intravenously into mice and their concentration in blood was determined by a fluorescence assay as a function of post-injection time. The elimination half-life (t 1/2) of CHPOA-PEGSH nanoparticles was about 15-fold longer (18h) than that of CHP nanogels (1.2h). The half-life enhancement of CHPOA-PEGSH was attributed to the presence of the crosslinker PEG chains, which prevent non-specific protein adsorption, and to the slow hydrolysis kinetics of the crosslinking esters in the biological milieu. The hybrid CHPOA-PEGSH nanogels are expected to be useful as injectable nanocarriers for drugs and proteins, in view of their low surface fouling and slow hydrolysis rate.
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U2 - 10.1016/j.colsurfb.2011.09.025
DO - 10.1016/j.colsurfb.2011.09.025
M3 - Article
C2 - 21996463
AN - SCOPUS:84863872137
SN - 0927-7765
VL - 99
SP - 38
EP - 44
JO - Colloids and Surfaces B: Biointerfaces
JF - Colloids and Surfaces B: Biointerfaces
ER -