Dual-immunohistochemistry provides little evidence for epithelial- mesenchymal transition in pulmonary fibrosis

Mizuho Yamada, Kazuyoshi Kuwano, Takashige Maeyama, Naoki Hamada, Michihiro Yoshimi, Yoichi Nakanishi, Michael Kasper

Research output: Contribution to journalArticle

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Abstract

epithelial-mesenchymal transition (EMT) has been considered to be involved in organ fibrogenesis. However, there is few direct evidence of this process in the pathophysiology of pulmonary fibrosis in vivo. Therefore, we tried to verify the involvement of this process in the development of pulmonary fibrosis. Since the co-expressions of epithelial and mesenchymal markers are thought to be a marker of EMT, we performed dual-immuunohistochemistry to assess the co-expressions of these proteins in lung tissues from bleomycin-induced pulmonary fibrosis in mice, and from patients with idiopathic pulmonary fibrosis, and nonspecific interstitial pneumonia. Double positive cells for epithelial markers including E-cadherin, T1α, or aquaporin 5, and a mesenchymal markers α-smooth muscle actin or vimentin were not found in bleomycin-induced pulmonary fibrosis in mice. Double positive cells for E-cadherin, ICAM-1, LEA, CD44v9, or SP-A and α-smooth muscle actin or vimentin were not found in lung tissues from normal lung parenchyma, idiopathic pulmonary fibrosis and nonspecific interstitial pneumonia. These results offer at least two possibilities. One is that EMT does not occur in IPF or bleomycin-induced pulmonary fibrosis in mice. Another is that EMT may occur in pulmonary fibrosis but the time during this transition in which cells express detectable levels of epithelial and mesenchymal markers is too small to be detected by double immunohistochemistry.

Original languageEnglish
Pages (from-to)453-462
Number of pages10
JournalHistochemistry and Cell Biology
Volume129
Issue number4
DOIs
Publication statusPublished - Apr 1 2008

Fingerprint

Epithelial-Mesenchymal Transition
Pulmonary Fibrosis
Immunohistochemistry
Cadherins
Idiopathic Pulmonary Fibrosis
Interstitial Lung Diseases
Bleomycin
Vimentin
Lung
Smooth Muscle
Actins
Aquaporin 5
Intercellular Adhesion Molecule-1
Epithelial Cells
Proteins

All Science Journal Classification (ASJC) codes

  • Histology
  • Molecular Biology
  • Medical Laboratory Technology
  • Cell Biology

Cite this

Dual-immunohistochemistry provides little evidence for epithelial- mesenchymal transition in pulmonary fibrosis. / Yamada, Mizuho; Kuwano, Kazuyoshi; Maeyama, Takashige; Hamada, Naoki; Yoshimi, Michihiro; Nakanishi, Yoichi; Kasper, Michael.

In: Histochemistry and Cell Biology, Vol. 129, No. 4, 01.04.2008, p. 453-462.

Research output: Contribution to journalArticle

Yamada, Mizuho ; Kuwano, Kazuyoshi ; Maeyama, Takashige ; Hamada, Naoki ; Yoshimi, Michihiro ; Nakanishi, Yoichi ; Kasper, Michael. / Dual-immunohistochemistry provides little evidence for epithelial- mesenchymal transition in pulmonary fibrosis. In: Histochemistry and Cell Biology. 2008 ; Vol. 129, No. 4. pp. 453-462.
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