Dual modulation by adenosine of ATP-activated channels through GTP-binding proteins in rat pheochromocytoma PC12 cells

Kazuhide Inoue, Tomokazu Watano, Shuichi Koizumi, Ken Nakazawa, Geoffrey Burnstock

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Abstract

Effects of adenosine on inward current activated by extracellular ATP were examined in rat pheochromocytoma PC12 cells. Adenosine induced two types of modulation on the current activated by 30 μM ATP; a low concentration of adenosine (1 μM) inhibited the current whereas a high concentration (>10 μM) enhanced the current. Neither the inhibition nor the enhancement was observed in cells pretreated with pertussis toxin (PTX), or in cells dialyzed with guanosine 5′-O-(2-thiotriphosphate) trilithium salt (GDPβS). In contrast, dialysis with K-252a, a protein kinase inhibitor, abolished the inhibition, but not the enhancement. Adenosine induced similar inhibition and enhancement on ATP-evoked increase in intracellular free Ca2+ concentration. The results suggest that adenosine produces dual modulation on the ATP-activated channels through different mechanisms involving PTX-sensitive GTP-binding proteins.

Original languageEnglish
Pages (from-to)223-229
Number of pages7
JournalEuropean Journal of Pharmacology: Molecular Pharmacology
Volume268
Issue number2
DOIs
Publication statusPublished - Jul 15 1994
Externally publishedYes

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All Science Journal Classification (ASJC) codes

  • Pharmacology

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