Dual regulatory effects of interferon-α, -β, and - γ on interleukin-8 gene expression by human gingival fibroblasts in culture upon stimulation with lipopolysaccharide from Prevotella intermedia, interleukin-1α, or tumor necrosis factor-α

T. Sakuta, M. Tokuda, M. Tamura, Eijiro Jimi, T. Ikebe, T. Koga, S. Nagaoka, H. Takada

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

In a previous study, we demonstrated that the amount of interleukin (IL)-8 mRNA expressed by human gingival fibroblasts stimulated with lipopolysaccharide (LPS) from Prevotella intermedia ATCC 25611 is increased by pre-treatment with beta or gamma interferon (IFN-β or -γ). In the present study, we identified the regulatory effects of these IFNs on IL-8 mRNA expression and IL-8 production by human gingival fibroblasts. Priming with IFN-alpha (α), -β, or -γ upregulated the IL-8 mRNA expression in response to P. intermedia LPS, whereas co-stimulation with these IFNs reduced the amount of mRNA expressed by the cells. The regulation of IL-8 mRNA expression induced by recombinant human tumor necrosis factor-α (rHuTNF-α) or rHuIL-1α was similar to that induced by LPS. The IL-8 mRNA expression in response to P. intermedia LPS was enhanced by IFN-γ independently of de novo protein synthesis, and was regulated, at least in part, at the transcriptional level. The IL-8 mRNA accumulation in response to P. intermedia LPS was inhibited by tosylphenyl-alanyl chloromethyl-ketone, an inhibitor of NF-κB activation, although the NF-κB activation itself was not altered by IFN-γ. These findings suggest that IFNs might be capable of both enhancing and inhibiting inflammatory responses in periodontal tissues through the dual regulation of IL-8 production by gingival fibroblasts in response to bacterial components and cytokines.

Original languageEnglish
Pages (from-to)1597-1605
Number of pages9
JournalJournal of Dental Research
Volume77
Issue number8
Publication statusPublished - Dec 1 1998
Externally publishedYes

Fingerprint

Prevotella intermedia
Interleukin-8
Interleukin-1
Interferons
Lipopolysaccharides
Tumor Necrosis Factor-alpha
Fibroblasts
Gene Expression
Messenger RNA
Interferon-beta
Ketones
Interferon-gamma
Cytokines

All Science Journal Classification (ASJC) codes

  • Dentistry(all)

Cite this

@article{9d6c7d1ae6704cdeb4202f9da3f8b3b5,
title = "Dual regulatory effects of interferon-α, -β, and - γ on interleukin-8 gene expression by human gingival fibroblasts in culture upon stimulation with lipopolysaccharide from Prevotella intermedia, interleukin-1α, or tumor necrosis factor-α",
abstract = "In a previous study, we demonstrated that the amount of interleukin (IL)-8 mRNA expressed by human gingival fibroblasts stimulated with lipopolysaccharide (LPS) from Prevotella intermedia ATCC 25611 is increased by pre-treatment with beta or gamma interferon (IFN-β or -γ). In the present study, we identified the regulatory effects of these IFNs on IL-8 mRNA expression and IL-8 production by human gingival fibroblasts. Priming with IFN-alpha (α), -β, or -γ upregulated the IL-8 mRNA expression in response to P. intermedia LPS, whereas co-stimulation with these IFNs reduced the amount of mRNA expressed by the cells. The regulation of IL-8 mRNA expression induced by recombinant human tumor necrosis factor-α (rHuTNF-α) or rHuIL-1α was similar to that induced by LPS. The IL-8 mRNA expression in response to P. intermedia LPS was enhanced by IFN-γ independently of de novo protein synthesis, and was regulated, at least in part, at the transcriptional level. The IL-8 mRNA accumulation in response to P. intermedia LPS was inhibited by tosylphenyl-alanyl chloromethyl-ketone, an inhibitor of NF-κB activation, although the NF-κB activation itself was not altered by IFN-γ. These findings suggest that IFNs might be capable of both enhancing and inhibiting inflammatory responses in periodontal tissues through the dual regulation of IL-8 production by gingival fibroblasts in response to bacterial components and cytokines.",
author = "T. Sakuta and M. Tokuda and M. Tamura and Eijiro Jimi and T. Ikebe and T. Koga and S. Nagaoka and H. Takada",
year = "1998",
month = "12",
day = "1",
language = "English",
volume = "77",
pages = "1597--1605",
journal = "Journal of Dental Research",
issn = "0022-0345",
publisher = "SAGE Publications Inc.",
number = "8",

}

TY - JOUR

T1 - Dual regulatory effects of interferon-α, -β, and - γ on interleukin-8 gene expression by human gingival fibroblasts in culture upon stimulation with lipopolysaccharide from Prevotella intermedia, interleukin-1α, or tumor necrosis factor-α

AU - Sakuta, T.

AU - Tokuda, M.

AU - Tamura, M.

AU - Jimi, Eijiro

AU - Ikebe, T.

AU - Koga, T.

AU - Nagaoka, S.

AU - Takada, H.

PY - 1998/12/1

Y1 - 1998/12/1

N2 - In a previous study, we demonstrated that the amount of interleukin (IL)-8 mRNA expressed by human gingival fibroblasts stimulated with lipopolysaccharide (LPS) from Prevotella intermedia ATCC 25611 is increased by pre-treatment with beta or gamma interferon (IFN-β or -γ). In the present study, we identified the regulatory effects of these IFNs on IL-8 mRNA expression and IL-8 production by human gingival fibroblasts. Priming with IFN-alpha (α), -β, or -γ upregulated the IL-8 mRNA expression in response to P. intermedia LPS, whereas co-stimulation with these IFNs reduced the amount of mRNA expressed by the cells. The regulation of IL-8 mRNA expression induced by recombinant human tumor necrosis factor-α (rHuTNF-α) or rHuIL-1α was similar to that induced by LPS. The IL-8 mRNA expression in response to P. intermedia LPS was enhanced by IFN-γ independently of de novo protein synthesis, and was regulated, at least in part, at the transcriptional level. The IL-8 mRNA accumulation in response to P. intermedia LPS was inhibited by tosylphenyl-alanyl chloromethyl-ketone, an inhibitor of NF-κB activation, although the NF-κB activation itself was not altered by IFN-γ. These findings suggest that IFNs might be capable of both enhancing and inhibiting inflammatory responses in periodontal tissues through the dual regulation of IL-8 production by gingival fibroblasts in response to bacterial components and cytokines.

AB - In a previous study, we demonstrated that the amount of interleukin (IL)-8 mRNA expressed by human gingival fibroblasts stimulated with lipopolysaccharide (LPS) from Prevotella intermedia ATCC 25611 is increased by pre-treatment with beta or gamma interferon (IFN-β or -γ). In the present study, we identified the regulatory effects of these IFNs on IL-8 mRNA expression and IL-8 production by human gingival fibroblasts. Priming with IFN-alpha (α), -β, or -γ upregulated the IL-8 mRNA expression in response to P. intermedia LPS, whereas co-stimulation with these IFNs reduced the amount of mRNA expressed by the cells. The regulation of IL-8 mRNA expression induced by recombinant human tumor necrosis factor-α (rHuTNF-α) or rHuIL-1α was similar to that induced by LPS. The IL-8 mRNA expression in response to P. intermedia LPS was enhanced by IFN-γ independently of de novo protein synthesis, and was regulated, at least in part, at the transcriptional level. The IL-8 mRNA accumulation in response to P. intermedia LPS was inhibited by tosylphenyl-alanyl chloromethyl-ketone, an inhibitor of NF-κB activation, although the NF-κB activation itself was not altered by IFN-γ. These findings suggest that IFNs might be capable of both enhancing and inhibiting inflammatory responses in periodontal tissues through the dual regulation of IL-8 production by gingival fibroblasts in response to bacterial components and cytokines.

UR - http://www.scopus.com/inward/record.url?scp=0032223607&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032223607&partnerID=8YFLogxK

M3 - Article

C2 - 9719033

AN - SCOPUS:0032223607

VL - 77

SP - 1597

EP - 1605

JO - Journal of Dental Research

JF - Journal of Dental Research

SN - 0022-0345

IS - 8

ER -