TY - JOUR
T1 - Dual role of the receptor Tom20 in specificity and efficiency of protein import into mitochondria
AU - Yamamoto, Hayashi
AU - Itoh, Nobuka
AU - Kawano, Shin
AU - Yatsukawa, Yoh Ichi
AU - Momose, Takaki
AU - Makio, Tadashi
AU - Matsunaga, Mayumi
AU - Yokota, Mihoko
AU - Esaki, Masatoshi
AU - Shodai, Toshihiro
AU - Kohda, Daisuke
AU - Aiken Hobbs, Alyson E.
AU - Jensen, Robert E.
AU - Endo, Toshiya
PY - 2011/1/4
Y1 - 2011/1/4
N2 - Mitochondria import most of their resident proteins from the cytosol, and the import receptor Tom20 of the outer-membrane translocator TOM40 complex plays an essential role in specificity of mitochondrial protein import. Here we analyzed the effects of Tom20 binding on NMR spectra of a long mitochondrial presequence and found that it contains two distinct Tom20-binding elements. In vitro import and cross-linking experiments revealed that, although the N-terminal Tom20-binding element is essential for targeting to mitochondria, the C-terminal element increases efficiency of protein import in the step prior to translocation across the inner membrane. Therefore Tom20 has a dual role in protein import into mitochondria: recognition of the targeting signal in the presequence and tethering the presequence to the TOM40 complex to increase import efficiency.
AB - Mitochondria import most of their resident proteins from the cytosol, and the import receptor Tom20 of the outer-membrane translocator TOM40 complex plays an essential role in specificity of mitochondrial protein import. Here we analyzed the effects of Tom20 binding on NMR spectra of a long mitochondrial presequence and found that it contains two distinct Tom20-binding elements. In vitro import and cross-linking experiments revealed that, although the N-terminal Tom20-binding element is essential for targeting to mitochondria, the C-terminal element increases efficiency of protein import in the step prior to translocation across the inner membrane. Therefore Tom20 has a dual role in protein import into mitochondria: recognition of the targeting signal in the presequence and tethering the presequence to the TOM40 complex to increase import efficiency.
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U2 - 10.1073/pnas.1014918108
DO - 10.1073/pnas.1014918108
M3 - Article
C2 - 21173275
AN - SCOPUS:78651083176
SN - 0027-8424
VL - 108
SP - 91
EP - 96
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 1
ER -