Dual specificity phosphatase16 is a negative regulator of c-Jun NH2-terminal kinase activity in T cells

Shino Kumabe, Momoe Itsumi, Hisakata Yamada, Toshiki Yajima, Tetsuya Matsuguchi, Yasunobu Yoshikai

    Research output: Contribution to journalArticle

    5 Citations (Scopus)

    Abstract

    Activation of MAPK is negatively regulated by DUSP, which dephosphorylate the phosphothreonine and phosphotyrosine residues. We have identified a novel JNK-specific DUSP, DUSP16, from murine macrophages. Its involvementinTcells has not yetbeendefined. Inthe present study,we found expression of DUSP16 in thymocytes and activated T cells but not in naive T cells. To elucidate the roles of DUSP16 in T cells, transgenic mice expressing a dominant negative form of DUSP16 specifically in T cells were generated (dnDUSP16 Tg). JNKactivity was selectively augmented in the thymocytes of these dnDUSP16 Tg mice. CD4 T cells in dnDUSP16 Tg mice showed normal levels of proliferation and IL-2 production after TCR triggering, while they produced increased IFN-γ but reduced Th2 cytokines compared with wild type CD4 T cells. On the other hand CD8 T cells in dnDUSP16 Tg mice produced an increased amount of IL-2, which resulted in enhanced proliferation and IFN-γ production. These results suggest that DUSP16 is an important regulator of JNK activity and effector functions of CD4 and CD8 T cells.

    Original languageEnglish
    Pages (from-to)105-111
    Number of pages7
    JournalMICROBIOLOGY and IMMUNOLOGY
    Volume54
    Issue number2
    DOIs
    Publication statusPublished - Feb 1 2010

    Fingerprint

    JNK Mitogen-Activated Protein Kinases
    T-Lymphocytes
    Thymocytes
    Interleukin-2
    Phosphothreonine
    Phosphotyrosine
    Transgenic Mice
    Macrophages
    Cytokines

    All Science Journal Classification (ASJC) codes

    • Microbiology
    • Immunology
    • Virology

    Cite this

    Dual specificity phosphatase16 is a negative regulator of c-Jun NH2-terminal kinase activity in T cells. / Kumabe, Shino; Itsumi, Momoe; Yamada, Hisakata; Yajima, Toshiki; Matsuguchi, Tetsuya; Yoshikai, Yasunobu.

    In: MICROBIOLOGY and IMMUNOLOGY, Vol. 54, No. 2, 01.02.2010, p. 105-111.

    Research output: Contribution to journalArticle

    Kumabe, Shino ; Itsumi, Momoe ; Yamada, Hisakata ; Yajima, Toshiki ; Matsuguchi, Tetsuya ; Yoshikai, Yasunobu. / Dual specificity phosphatase16 is a negative regulator of c-Jun NH2-terminal kinase activity in T cells. In: MICROBIOLOGY and IMMUNOLOGY. 2010 ; Vol. 54, No. 2. pp. 105-111.
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