Dynamic analysis of photosynthate translocation into strawberry fruits using non-invasive 11 C-labeling supported with conventional destructive measurements using 13 C-labeling

Kota Hidaka, Yuta Miyoshi, Satomi Ishii, Nobuo Suzui, Yong Gen Yin, Keisuke Kurita, Koyo Nagao, Takuya Araki, Daisuke Yasutake, Kitano Masaharu, Naoki Kawachi

Research output: Contribution to journalArticle

Abstract

In protected strawberry (Fragaria × ananassa Duch.) cultivation, environmental control based on the process of photosynthate translocation is essential for optimizing fruit quality and yield, because the process of photosynthate translocation directly affects dry matter partitioning. We visualized photosynthate translocation to strawberry fruits non-invasively with 11 CO 2 and a positron-emitting tracer imaging system (PETIS). We used PETIS to evaluate real-time dynamics of 11 C-labeled photosynthate translocation from a 11 CO 2 -fed leaf, which was immediately below the inflorescence, to individual fruits on an inflorescence in intact plant. Serial photosynthate translocation images and animations obtained by PETIS verified that the 11 C-photosynthates from the source leaf reached the sink fruit within 1 h but did not accumulate homogeneously within a fruit. The quantity of photosynthate translocation as represented by 11 C radioactivity varied among individual fruits and their positions on the inflorescence. Photosynthate translocation rates to secondary fruit were faster than those to primary or tertiary fruits, even though the translocation pathway from leaf to fruit was the longest for the secondary fruit. Moreover, the secondary fruit was 25% smaller than the primary fruit. Sink activity ( 11 C radioactivity/dry weight [DW]) of the secondary fruit was higher than those of the primary and tertiary fruits. These relative differences in sink activity levels among the three fruit positions were also confirmed by 13 C tracer measurement. Photosynthate translocation rates in the pedicels might be dependent on the sink strength of the adjoining fruits. The present study established 11 C-photosynthate arrival times to the sink fruits and demonstrated that the translocated material does not uniformly accumulate within a fruit. The actual quantities of translocated photosynthates from a specific leaf differed among individual fruits on the same inflorescence. To the best of our knowledge, this is the first reported observation of real-time translocation to individual fruits in an intact strawberry plant using 11 C-radioactive-and 13 C-stable-isotope analyses.

Original languageEnglish
Article number1946
JournalFrontiers in Plant Science
Volume9
DOIs
Publication statusPublished - Jan 9 2019

Fingerprint

fruits
photosynthates
dynamic analysis
translocation
marking
strawberries
fruit
sinks
leaves
tracer techniques
tracers
inflorescences
tracer
labelling
positrons
electrons
image analysis
radioactivity
environmental control
animation

All Science Journal Classification (ASJC) codes

  • Plant Science

Cite this

Dynamic analysis of photosynthate translocation into strawberry fruits using non-invasive 11 C-labeling supported with conventional destructive measurements using 13 C-labeling . / Hidaka, Kota; Miyoshi, Yuta; Ishii, Satomi; Suzui, Nobuo; Yin, Yong Gen; Kurita, Keisuke; Nagao, Koyo; Araki, Takuya; Yasutake, Daisuke; Masaharu, Kitano; Kawachi, Naoki.

In: Frontiers in Plant Science, Vol. 9, 1946, 09.01.2019.

Research output: Contribution to journalArticle

Hidaka, Kota ; Miyoshi, Yuta ; Ishii, Satomi ; Suzui, Nobuo ; Yin, Yong Gen ; Kurita, Keisuke ; Nagao, Koyo ; Araki, Takuya ; Yasutake, Daisuke ; Masaharu, Kitano ; Kawachi, Naoki. / Dynamic analysis of photosynthate translocation into strawberry fruits using non-invasive 11 C-labeling supported with conventional destructive measurements using 13 C-labeling In: Frontiers in Plant Science. 2019 ; Vol. 9.
@article{27b634136d164151b25aa6cfdfedf0c6,
title = "Dynamic analysis of photosynthate translocation into strawberry fruits using non-invasive 11 C-labeling supported with conventional destructive measurements using 13 C-labeling",
abstract = "In protected strawberry (Fragaria × ananassa Duch.) cultivation, environmental control based on the process of photosynthate translocation is essential for optimizing fruit quality and yield, because the process of photosynthate translocation directly affects dry matter partitioning. We visualized photosynthate translocation to strawberry fruits non-invasively with 11 CO 2 and a positron-emitting tracer imaging system (PETIS). We used PETIS to evaluate real-time dynamics of 11 C-labeled photosynthate translocation from a 11 CO 2 -fed leaf, which was immediately below the inflorescence, to individual fruits on an inflorescence in intact plant. Serial photosynthate translocation images and animations obtained by PETIS verified that the 11 C-photosynthates from the source leaf reached the sink fruit within 1 h but did not accumulate homogeneously within a fruit. The quantity of photosynthate translocation as represented by 11 C radioactivity varied among individual fruits and their positions on the inflorescence. Photosynthate translocation rates to secondary fruit were faster than those to primary or tertiary fruits, even though the translocation pathway from leaf to fruit was the longest for the secondary fruit. Moreover, the secondary fruit was 25{\%} smaller than the primary fruit. Sink activity ( 11 C radioactivity/dry weight [DW]) of the secondary fruit was higher than those of the primary and tertiary fruits. These relative differences in sink activity levels among the three fruit positions were also confirmed by 13 C tracer measurement. Photosynthate translocation rates in the pedicels might be dependent on the sink strength of the adjoining fruits. The present study established 11 C-photosynthate arrival times to the sink fruits and demonstrated that the translocated material does not uniformly accumulate within a fruit. The actual quantities of translocated photosynthates from a specific leaf differed among individual fruits on the same inflorescence. To the best of our knowledge, this is the first reported observation of real-time translocation to individual fruits in an intact strawberry plant using 11 C-radioactive-and 13 C-stable-isotope analyses.",
author = "Kota Hidaka and Yuta Miyoshi and Satomi Ishii and Nobuo Suzui and Yin, {Yong Gen} and Keisuke Kurita and Koyo Nagao and Takuya Araki and Daisuke Yasutake and Kitano Masaharu and Naoki Kawachi",
year = "2019",
month = "1",
day = "9",
doi = "10.3389/fpls.2018.01946",
language = "English",
volume = "9",
journal = "Frontiers in Plant Science",
issn = "1664-462X",
publisher = "Frontiers Media S. A.",

}

TY - JOUR

T1 - Dynamic analysis of photosynthate translocation into strawberry fruits using non-invasive 11 C-labeling supported with conventional destructive measurements using 13 C-labeling

AU - Hidaka, Kota

AU - Miyoshi, Yuta

AU - Ishii, Satomi

AU - Suzui, Nobuo

AU - Yin, Yong Gen

AU - Kurita, Keisuke

AU - Nagao, Koyo

AU - Araki, Takuya

AU - Yasutake, Daisuke

AU - Masaharu, Kitano

AU - Kawachi, Naoki

PY - 2019/1/9

Y1 - 2019/1/9

N2 - In protected strawberry (Fragaria × ananassa Duch.) cultivation, environmental control based on the process of photosynthate translocation is essential for optimizing fruit quality and yield, because the process of photosynthate translocation directly affects dry matter partitioning. We visualized photosynthate translocation to strawberry fruits non-invasively with 11 CO 2 and a positron-emitting tracer imaging system (PETIS). We used PETIS to evaluate real-time dynamics of 11 C-labeled photosynthate translocation from a 11 CO 2 -fed leaf, which was immediately below the inflorescence, to individual fruits on an inflorescence in intact plant. Serial photosynthate translocation images and animations obtained by PETIS verified that the 11 C-photosynthates from the source leaf reached the sink fruit within 1 h but did not accumulate homogeneously within a fruit. The quantity of photosynthate translocation as represented by 11 C radioactivity varied among individual fruits and their positions on the inflorescence. Photosynthate translocation rates to secondary fruit were faster than those to primary or tertiary fruits, even though the translocation pathway from leaf to fruit was the longest for the secondary fruit. Moreover, the secondary fruit was 25% smaller than the primary fruit. Sink activity ( 11 C radioactivity/dry weight [DW]) of the secondary fruit was higher than those of the primary and tertiary fruits. These relative differences in sink activity levels among the three fruit positions were also confirmed by 13 C tracer measurement. Photosynthate translocation rates in the pedicels might be dependent on the sink strength of the adjoining fruits. The present study established 11 C-photosynthate arrival times to the sink fruits and demonstrated that the translocated material does not uniformly accumulate within a fruit. The actual quantities of translocated photosynthates from a specific leaf differed among individual fruits on the same inflorescence. To the best of our knowledge, this is the first reported observation of real-time translocation to individual fruits in an intact strawberry plant using 11 C-radioactive-and 13 C-stable-isotope analyses.

AB - In protected strawberry (Fragaria × ananassa Duch.) cultivation, environmental control based on the process of photosynthate translocation is essential for optimizing fruit quality and yield, because the process of photosynthate translocation directly affects dry matter partitioning. We visualized photosynthate translocation to strawberry fruits non-invasively with 11 CO 2 and a positron-emitting tracer imaging system (PETIS). We used PETIS to evaluate real-time dynamics of 11 C-labeled photosynthate translocation from a 11 CO 2 -fed leaf, which was immediately below the inflorescence, to individual fruits on an inflorescence in intact plant. Serial photosynthate translocation images and animations obtained by PETIS verified that the 11 C-photosynthates from the source leaf reached the sink fruit within 1 h but did not accumulate homogeneously within a fruit. The quantity of photosynthate translocation as represented by 11 C radioactivity varied among individual fruits and their positions on the inflorescence. Photosynthate translocation rates to secondary fruit were faster than those to primary or tertiary fruits, even though the translocation pathway from leaf to fruit was the longest for the secondary fruit. Moreover, the secondary fruit was 25% smaller than the primary fruit. Sink activity ( 11 C radioactivity/dry weight [DW]) of the secondary fruit was higher than those of the primary and tertiary fruits. These relative differences in sink activity levels among the three fruit positions were also confirmed by 13 C tracer measurement. Photosynthate translocation rates in the pedicels might be dependent on the sink strength of the adjoining fruits. The present study established 11 C-photosynthate arrival times to the sink fruits and demonstrated that the translocated material does not uniformly accumulate within a fruit. The actual quantities of translocated photosynthates from a specific leaf differed among individual fruits on the same inflorescence. To the best of our knowledge, this is the first reported observation of real-time translocation to individual fruits in an intact strawberry plant using 11 C-radioactive-and 13 C-stable-isotope analyses.

UR - http://www.scopus.com/inward/record.url?scp=85062690455&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85062690455&partnerID=8YFLogxK

U2 - 10.3389/fpls.2018.01946

DO - 10.3389/fpls.2018.01946

M3 - Article

VL - 9

JO - Frontiers in Plant Science

JF - Frontiers in Plant Science

SN - 1664-462X

M1 - 1946

ER -