Abstract
We report a dynamic cross-linking effect of Mg2+ that enhances the sieving properties of low-viscosity poly-(vinylpyrrolidone) (PVP) solutions. A low-viscosity PVP solution was applied to nondenaturing microchip electrophoresis of protein samples using microchips made of poly(methyl methacrylate). The separation resolution of nondenatured protein markers in 1.8% PVP solution was improved by adding 1-20 mM MgCl2. We studied the effect of the ratio of cross-linking agent on mobility of protein samples and showed that protein retardation (In μ/μ0) is correlated with the ratio of cross-linking agent to PVP ([cMg2+/cPVP]) as In μ/μ0 = A′[cMg2+ /cPVP] b′. A′ was related to the protein radius (R), and b′ was found to be 0.72 for proteins with R = 2.4 nm and 0.82 for proteins with R = 1.85 nm. A structural study of PVP in semidilute solutions using dynamic light scattering showed that incremental increases of Mg2+ ion concentration from 5 to 20 mM in 1.8% PVP solution increased the hydrodynamic radius of PVP polymers by 20%.
| Original language | English |
|---|---|
| Pages (from-to) | 312-316 |
| Number of pages | 5 |
| Journal | Analytical Chemistry |
| Volume | 80 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Jan 1 2008 |
| Externally published | Yes |
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All Science Journal Classification (ASJC) codes
- Analytical Chemistry
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Dynamic cross-linking effect of Mg2+ to enhance sieving properties of low-viscosity poly(vinylpyrrolidone) solutions for microchip electrophoresis of proteins. / Mohamadi, Mohamad Reza; Kaji, Noritada; Tokeshi, Anabu; Baba, Yoshinobu.
In: Analytical Chemistry, Vol. 80, No. 1, 01.01.2008, p. 312-316.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Dynamic cross-linking effect of Mg2+ to enhance sieving properties of low-viscosity poly(vinylpyrrolidone) solutions for microchip electrophoresis of proteins
AU - Mohamadi, Mohamad Reza
AU - Kaji, Noritada
AU - Tokeshi, Anabu
AU - Baba, Yoshinobu
PY - 2008/1/1
Y1 - 2008/1/1
N2 - We report a dynamic cross-linking effect of Mg2+ that enhances the sieving properties of low-viscosity poly-(vinylpyrrolidone) (PVP) solutions. A low-viscosity PVP solution was applied to nondenaturing microchip electrophoresis of protein samples using microchips made of poly(methyl methacrylate). The separation resolution of nondenatured protein markers in 1.8% PVP solution was improved by adding 1-20 mM MgCl2. We studied the effect of the ratio of cross-linking agent on mobility of protein samples and showed that protein retardation (In μ/μ0) is correlated with the ratio of cross-linking agent to PVP ([cMg2+/cPVP]) as In μ/μ0 = A′[cMg2+ /cPVP] b′. A′ was related to the protein radius (R), and b′ was found to be 0.72 for proteins with R = 2.4 nm and 0.82 for proteins with R = 1.85 nm. A structural study of PVP in semidilute solutions using dynamic light scattering showed that incremental increases of Mg2+ ion concentration from 5 to 20 mM in 1.8% PVP solution increased the hydrodynamic radius of PVP polymers by 20%.
AB - We report a dynamic cross-linking effect of Mg2+ that enhances the sieving properties of low-viscosity poly-(vinylpyrrolidone) (PVP) solutions. A low-viscosity PVP solution was applied to nondenaturing microchip electrophoresis of protein samples using microchips made of poly(methyl methacrylate). The separation resolution of nondenatured protein markers in 1.8% PVP solution was improved by adding 1-20 mM MgCl2. We studied the effect of the ratio of cross-linking agent on mobility of protein samples and showed that protein retardation (In μ/μ0) is correlated with the ratio of cross-linking agent to PVP ([cMg2+/cPVP]) as In μ/μ0 = A′[cMg2+ /cPVP] b′. A′ was related to the protein radius (R), and b′ was found to be 0.72 for proteins with R = 2.4 nm and 0.82 for proteins with R = 1.85 nm. A structural study of PVP in semidilute solutions using dynamic light scattering showed that incremental increases of Mg2+ ion concentration from 5 to 20 mM in 1.8% PVP solution increased the hydrodynamic radius of PVP polymers by 20%.
UR - http://www.scopus.com/inward/record.url?scp=41149134019&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=41149134019&partnerID=8YFLogxK
U2 - 10.1021/ac701974u
DO - 10.1021/ac701974u
M3 - Article
C2 - 18052344
AN - SCOPUS:41149134019
VL - 80
SP - 312
EP - 316
JO - Analytical Chemistry
JF - Analytical Chemistry
SN - 0003-2700
IS - 1
ER -