Dynamic expression of Sox2, Gata3, and Prox1 during primary auditory neuron development in the mammalian cochlea

Koji Nishimura, Teppei Noda, Alain Dabdoub

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Primary auditory neurons (PANs) connect cochlear sensory hair cells in the mammalian inner ear to cochlear nucleus neurons in the brainstem. PANs develop from neuroblasts delaminated from the proneurosensory domain of the otocyst and keep maturing until the onset of hearing after birth. There are two types of PANs: type I, which innervate the inner hair cells (IHCs), and type II, which innervate the outer hair cells (OHCs). Glial cells surrounding these neurons originate from neural crest cells and migrate to the spiral ganglion. Several transcription factors are known to regulate the development and differentiation of PANs. Here we systematically examined the spatiotemporal expression of five transcription factors: Sox2, Sox10, Gata3, Mafb, and Prox1 from early delamination at embryonic day (E) 10.5 to adult. We found that Sox2 and Sox10 were initially expressed in the proneurosensory cells in the otocyst (E10.5). By E12.75 both Sox2 and Sox10 were downregulated in the developing PANs; however, Sox2 expression transiently increased in the neurons around birth. Furthermore, both Sox2 and Sox10 continued to be expressed in spiral ganglion glial cells. We also show that Gata3 and Prox1 were first expressed in all developing neurons, followed by a decrease in expression of Gata3 and Mafb in type I PANs and Prox1 in type II PANs as they matured. Moreover, we describe two subtypes of type II neurons based on Peripherin expression. These results suggest that Sox2, Gata3 and Prox1 play a role during neurogenesis as well as maturation of the PANs.

Original languageEnglish
Article numbere0170568
JournalPloS one
Volume12
Issue number1
DOIs
Publication statusPublished - Jan 2017

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Cochlea
Neurons
neurons
Spiral Ganglion
hairs
Cells
neuroglia
Outer Auditory Hair Cells
Inner Auditory Hair Cells
Neuroglia
cells
Transcription Factors
Auditory Hair Cells
transcription factors
Peripherins
Parturition
Cochlear Nucleus
neural crest
neurogenesis
Neural Crest

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Dynamic expression of Sox2, Gata3, and Prox1 during primary auditory neuron development in the mammalian cochlea. / Nishimura, Koji; Noda, Teppei; Dabdoub, Alain.

In: PloS one, Vol. 12, No. 1, e0170568, 01.2017.

Research output: Contribution to journalArticle

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