Dynamic metabolic profiling of cyanobacterial glycogen biosynthesis under conditions of nitrate depletion

Tomohisa Hasunuma, Fumi Kikuyama, Mami Matsuda, Shimpei Aikawa, Yoshihiro Izumi, Akihiko Kondo

Research output: Contribution to journalArticle

79 Citations (Scopus)

Abstract

Cyanobacteria represent a globally important biomass because they are responsible for a substantial proportion of primary production in the hydrosphere. Arthrospira platensis is a fast-growing halophilic cyanobacterium capable of accumulating glycogen and has the potential to serve as a feedstock in the fermentative production of third-generation biofuels. Accordingly, enhancing cyanobacterial glycogen production is a promising biofuel production strategy. However, the regulatory mechanism of glycogen metabolism in cyanobacteria is poorly understood. The aim of the present study was to determine the metabolic flux of glycogen biosynthesis using a dynamic metabolomic approach. Time-course profiling of widely targeted cyanobacterial metabolic intermediates demonstrated a global metabolic reprogramming that involves transient increases in the levels of some amino acids during the glycogen production phase induced by nitrate depletion. Also, in vivo labelling with NaH13CO3 enabled direct measurement of metabolic intermediate turnover in A. platensis, revealing that under conditions of nitrate depletion glycogen is biosynthesized with carbon derived from amino acids released from proteins via gluconeogenesis. This dynamic metabolic profiling approach provided conclusive evidence of temporal alterations in the metabolic profile in cyanobacterial cells.

Original languageEnglish
Pages (from-to)2943-2954
Number of pages12
JournalJournal of Experimental Botany
Volume64
Issue number10
DOIs
Publication statusPublished - Jul 1 2013
Externally publishedYes

Fingerprint

metabolomics
Glycogen
Nitrates
glycogen
nitrates
biosynthesis
Cyanobacteria
Spirulina platensis
Biofuels
biofuels
Spirulina
Amino Acids
amino acids
Metabolomics
Gluconeogenesis
Metabolome
gluconeogenesis
feedstocks
Biomass
Carbon

All Science Journal Classification (ASJC) codes

  • Physiology
  • Plant Science

Cite this

Dynamic metabolic profiling of cyanobacterial glycogen biosynthesis under conditions of nitrate depletion. / Hasunuma, Tomohisa; Kikuyama, Fumi; Matsuda, Mami; Aikawa, Shimpei; Izumi, Yoshihiro; Kondo, Akihiko.

In: Journal of Experimental Botany, Vol. 64, No. 10, 01.07.2013, p. 2943-2954.

Research output: Contribution to journalArticle

Hasunuma, Tomohisa ; Kikuyama, Fumi ; Matsuda, Mami ; Aikawa, Shimpei ; Izumi, Yoshihiro ; Kondo, Akihiko. / Dynamic metabolic profiling of cyanobacterial glycogen biosynthesis under conditions of nitrate depletion. In: Journal of Experimental Botany. 2013 ; Vol. 64, No. 10. pp. 2943-2954.
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