Dynamic modification of poly(methyl methacrylate) chips using poly(vinyl alcohol) for glycosaminoglycan disaccharide isomer separation

Yong Zhang, Guichen Ping, Noritada Kaji, Manabu Tokeshi, Yoshi Baba

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

We describe a microchip electrophoresis (MCE) method for the assay of unsaturated disaccharides of chondroitin sulfates, dermatan sulfates, and hyaluronic acid (HA). Poly(vinyl alcohol) (PVA) could be irreversibly adsorbed onto poly(methyl methacrylate) (PMMA) substrates and this approach was applicable for dynamic coating. The characteristics of the PMMA surface with PVA coating were evaluated in terms of the wettability, EOF, and adsorption of 2-aminoacridone (AMAC)-labeled disaccharide. The water contact angle decreased from ∼73° on a pristine PMMA surface to ∼37.5° on a PVA-coated surface, indicating that the PVA coating increased hydrophilicity. EOF was reduced approximately twofold and was relatively stable. Scanning electron microscopy and fluorescence microscopy images showed that adsorption of AMAC-labeled disaccharides was dramatically suppressed. Using the PVA coating, baseline separation of two pairs of glycosaminoglycan (GAG) disaccharide isomers, ΔDi-diSB/ΔDi-diSD and ΔDi-0S/ΔDi-HA, was achieved in Tris-borate buffer within 130 s by MCE.

Original languageEnglish
Pages (from-to)3308-3314
Number of pages7
JournalELECTROPHORESIS
Volume28
Issue number18
DOIs
Publication statusPublished - Sep 2007
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry

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