TY - JOUR
T1 - Dynamic regulation of intracellular Ca2+ concentration in arotic endothelial cells
AU - Oike, Masahiro
AU - Ito, Yushi
N1 - Funding Information:
We thank Dr. K. Creed for critical reading of the manuscript. This work was supported in part by a grant-in-aid from the Ministry of Education of Japan.
PY - 1997/1/29
Y1 - 1997/1/29
N2 - In non-excitable cells, a Ca2+ entry pathway is opened after the depletion of intracellular Ca2+ store sites. We have tried to estimate ATP released almost all stored Ca2+ in Ca2+-free extracellular solution, whereas a low concentration of ATP (30 nM) produced a partial (57.3 ± 3.0%) release of Ca2+. By 10 min of Ca2+ re-perfusion, the Ca2+ store site was reloaded to 97.1% of its initial filling state. When thapsigargin was applied to this cell in Mn2+ solution, Mn2+-induced quenching of fura-2 dye started when 19.3 ± 5.3% of Ca2+ release, produced by 30 nM ATP, had occurred. Therefore, Ca2+ release required for Mn2+ entry was estimated as 11.1 C 3.0% of stored Ca2+. These results indicate that intracellular Ca2+ concentration is controlled dynamically by simultaneously occurring Ca2+ release and entry in bovine aortic endothelial cells.
AB - In non-excitable cells, a Ca2+ entry pathway is opened after the depletion of intracellular Ca2+ store sites. We have tried to estimate ATP released almost all stored Ca2+ in Ca2+-free extracellular solution, whereas a low concentration of ATP (30 nM) produced a partial (57.3 ± 3.0%) release of Ca2+. By 10 min of Ca2+ re-perfusion, the Ca2+ store site was reloaded to 97.1% of its initial filling state. When thapsigargin was applied to this cell in Mn2+ solution, Mn2+-induced quenching of fura-2 dye started when 19.3 ± 5.3% of Ca2+ release, produced by 30 nM ATP, had occurred. Therefore, Ca2+ release required for Mn2+ entry was estimated as 11.1 C 3.0% of stored Ca2+. These results indicate that intracellular Ca2+ concentration is controlled dynamically by simultaneously occurring Ca2+ release and entry in bovine aortic endothelial cells.
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U2 - 10.1016/S0014-2999(96)00846-1
DO - 10.1016/S0014-2999(96)00846-1
M3 - Article
C2 - 9042604
AN - SCOPUS:0031033182
VL - 319
SP - 291
EP - 298
JO - European Journal of Pharmacology
JF - European Journal of Pharmacology
SN - 0014-2999
IS - 2-3
ER -