Dynamic regulation of intracellular Ca2+ concentration in arotic endothelial cells

Masahiro Oike, Yushi Ito

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

In non-excitable cells, a Ca2+ entry pathway is opened after the depletion of intracellular Ca2+ store sites. We have tried to estimate ATP released almost all stored Ca2+ in Ca2+-free extracellular solution, whereas a low concentration of ATP (30 nM) produced a partial (57.3 ± 3.0%) release of Ca2+. By 10 min of Ca2+ re-perfusion, the Ca2+ store site was reloaded to 97.1% of its initial filling state. When thapsigargin was applied to this cell in Mn2+ solution, Mn2+-induced quenching of fura-2 dye started when 19.3 ± 5.3% of Ca2+ release, produced by 30 nM ATP, had occurred. Therefore, Ca2+ release required for Mn2+ entry was estimated as 11.1 C 3.0% of stored Ca2+. These results indicate that intracellular Ca2+ concentration is controlled dynamically by simultaneously occurring Ca2+ release and entry in bovine aortic endothelial cells.

Original languageEnglish
Pages (from-to)291-298
Number of pages8
JournalEuropean Journal of Pharmacology
Volume319
Issue number2-3
DOIs
Publication statusPublished - Jan 29 1997

Fingerprint

Endothelial Cells
Adenosine Triphosphate
Thapsigargin
Fura-2
Coloring Agents
Perfusion

All Science Journal Classification (ASJC) codes

  • Pharmacology

Cite this

Dynamic regulation of intracellular Ca2+ concentration in arotic endothelial cells. / Oike, Masahiro; Ito, Yushi.

In: European Journal of Pharmacology, Vol. 319, No. 2-3, 29.01.1997, p. 291-298.

Research output: Contribution to journalArticle

@article{b59114591a044759bf195206d7b163a6,
title = "Dynamic regulation of intracellular Ca2+ concentration in arotic endothelial cells",
abstract = "In non-excitable cells, a Ca2+ entry pathway is opened after the depletion of intracellular Ca2+ store sites. We have tried to estimate ATP released almost all stored Ca2+ in Ca2+-free extracellular solution, whereas a low concentration of ATP (30 nM) produced a partial (57.3 ± 3.0{\%}) release of Ca2+. By 10 min of Ca2+ re-perfusion, the Ca2+ store site was reloaded to 97.1{\%} of its initial filling state. When thapsigargin was applied to this cell in Mn2+ solution, Mn2+-induced quenching of fura-2 dye started when 19.3 ± 5.3{\%} of Ca2+ release, produced by 30 nM ATP, had occurred. Therefore, Ca2+ release required for Mn2+ entry was estimated as 11.1 C 3.0{\%} of stored Ca2+. These results indicate that intracellular Ca2+ concentration is controlled dynamically by simultaneously occurring Ca2+ release and entry in bovine aortic endothelial cells.",
author = "Masahiro Oike and Yushi Ito",
year = "1997",
month = "1",
day = "29",
doi = "10.1016/S0014-2999(96)00846-1",
language = "English",
volume = "319",
pages = "291--298",
journal = "European Journal of Pharmacology",
issn = "0014-2999",
publisher = "Elsevier",
number = "2-3",

}

TY - JOUR

T1 - Dynamic regulation of intracellular Ca2+ concentration in arotic endothelial cells

AU - Oike, Masahiro

AU - Ito, Yushi

PY - 1997/1/29

Y1 - 1997/1/29

N2 - In non-excitable cells, a Ca2+ entry pathway is opened after the depletion of intracellular Ca2+ store sites. We have tried to estimate ATP released almost all stored Ca2+ in Ca2+-free extracellular solution, whereas a low concentration of ATP (30 nM) produced a partial (57.3 ± 3.0%) release of Ca2+. By 10 min of Ca2+ re-perfusion, the Ca2+ store site was reloaded to 97.1% of its initial filling state. When thapsigargin was applied to this cell in Mn2+ solution, Mn2+-induced quenching of fura-2 dye started when 19.3 ± 5.3% of Ca2+ release, produced by 30 nM ATP, had occurred. Therefore, Ca2+ release required for Mn2+ entry was estimated as 11.1 C 3.0% of stored Ca2+. These results indicate that intracellular Ca2+ concentration is controlled dynamically by simultaneously occurring Ca2+ release and entry in bovine aortic endothelial cells.

AB - In non-excitable cells, a Ca2+ entry pathway is opened after the depletion of intracellular Ca2+ store sites. We have tried to estimate ATP released almost all stored Ca2+ in Ca2+-free extracellular solution, whereas a low concentration of ATP (30 nM) produced a partial (57.3 ± 3.0%) release of Ca2+. By 10 min of Ca2+ re-perfusion, the Ca2+ store site was reloaded to 97.1% of its initial filling state. When thapsigargin was applied to this cell in Mn2+ solution, Mn2+-induced quenching of fura-2 dye started when 19.3 ± 5.3% of Ca2+ release, produced by 30 nM ATP, had occurred. Therefore, Ca2+ release required for Mn2+ entry was estimated as 11.1 C 3.0% of stored Ca2+. These results indicate that intracellular Ca2+ concentration is controlled dynamically by simultaneously occurring Ca2+ release and entry in bovine aortic endothelial cells.

UR - http://www.scopus.com/inward/record.url?scp=0031033182&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031033182&partnerID=8YFLogxK

U2 - 10.1016/S0014-2999(96)00846-1

DO - 10.1016/S0014-2999(96)00846-1

M3 - Article

C2 - 9042604

AN - SCOPUS:0031033182

VL - 319

SP - 291

EP - 298

JO - European Journal of Pharmacology

JF - European Journal of Pharmacology

SN - 0014-2999

IS - 2-3

ER -