Abstract
In non-excitable cells, a Ca2+ entry pathway is opened after the depletion of intracellular Ca2+ store sites. We have tried to estimate ATP released almost all stored Ca2+ in Ca2+-free extracellular solution, whereas a low concentration of ATP (30 nM) produced a partial (57.3 ± 3.0%) release of Ca2+. By 10 min of Ca2+ re-perfusion, the Ca2+ store site was reloaded to 97.1% of its initial filling state. When thapsigargin was applied to this cell in Mn2+ solution, Mn2+-induced quenching of fura-2 dye started when 19.3 ± 5.3% of Ca2+ release, produced by 30 nM ATP, had occurred. Therefore, Ca2+ release required for Mn2+ entry was estimated as 11.1 C 3.0% of stored Ca2+. These results indicate that intracellular Ca2+ concentration is controlled dynamically by simultaneously occurring Ca2+ release and entry in bovine aortic endothelial cells.
| Original language | English |
|---|---|
| Pages (from-to) | 291-298 |
| Number of pages | 8 |
| Journal | European Journal of Pharmacology |
| Volume | 319 |
| Issue number | 2-3 |
| DOIs | |
| Publication status | Published - Jan 29 1997 |
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All Science Journal Classification (ASJC) codes
- Pharmacology
Cite this
Dynamic regulation of intracellular Ca2+ concentration in arotic endothelial cells. / Oike, Masahiro; Ito, Yushi.
In: European Journal of Pharmacology, Vol. 319, No. 2-3, 29.01.1997, p. 291-298.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Dynamic regulation of intracellular Ca2+ concentration in arotic endothelial cells
AU - Oike, Masahiro
AU - Ito, Yushi
PY - 1997/1/29
Y1 - 1997/1/29
N2 - In non-excitable cells, a Ca2+ entry pathway is opened after the depletion of intracellular Ca2+ store sites. We have tried to estimate ATP released almost all stored Ca2+ in Ca2+-free extracellular solution, whereas a low concentration of ATP (30 nM) produced a partial (57.3 ± 3.0%) release of Ca2+. By 10 min of Ca2+ re-perfusion, the Ca2+ store site was reloaded to 97.1% of its initial filling state. When thapsigargin was applied to this cell in Mn2+ solution, Mn2+-induced quenching of fura-2 dye started when 19.3 ± 5.3% of Ca2+ release, produced by 30 nM ATP, had occurred. Therefore, Ca2+ release required for Mn2+ entry was estimated as 11.1 C 3.0% of stored Ca2+. These results indicate that intracellular Ca2+ concentration is controlled dynamically by simultaneously occurring Ca2+ release and entry in bovine aortic endothelial cells.
AB - In non-excitable cells, a Ca2+ entry pathway is opened after the depletion of intracellular Ca2+ store sites. We have tried to estimate ATP released almost all stored Ca2+ in Ca2+-free extracellular solution, whereas a low concentration of ATP (30 nM) produced a partial (57.3 ± 3.0%) release of Ca2+. By 10 min of Ca2+ re-perfusion, the Ca2+ store site was reloaded to 97.1% of its initial filling state. When thapsigargin was applied to this cell in Mn2+ solution, Mn2+-induced quenching of fura-2 dye started when 19.3 ± 5.3% of Ca2+ release, produced by 30 nM ATP, had occurred. Therefore, Ca2+ release required for Mn2+ entry was estimated as 11.1 C 3.0% of stored Ca2+. These results indicate that intracellular Ca2+ concentration is controlled dynamically by simultaneously occurring Ca2+ release and entry in bovine aortic endothelial cells.
UR - http://www.scopus.com/inward/record.url?scp=0031033182&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0031033182&partnerID=8YFLogxK
U2 - 10.1016/S0014-2999(96)00846-1
DO - 10.1016/S0014-2999(96)00846-1
M3 - Article
C2 - 9042604
AN - SCOPUS:0031033182
VL - 319
SP - 291
EP - 298
JO - European Journal of Pharmacology
JF - European Journal of Pharmacology
SN - 0014-2999
IS - 2-3
ER -