Dynamics of mitochondrial DNA nucleoids regulated by mitochondrial fission is essential for maintenance of homogeneously active mitochondria during neonatal heart development

Takaya Ishihara, Reiko Ban-Ishihara, Maki Maeda, Yui Matsunaga, Ayaka Ichimura, Sachiko Kyogoku, Hiroki Aoki, Shun Katada, Kazuto Nakada, Masatoshi Nomura, Noboru Mizushima, Katsuyoshi Mihara, Naotada Ishihara

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72 Citations (Scopus)

Abstract

Mitochondria are dynamic organelles, and their fusion and fission regulate cellular signaling, development, and mitochondrial homeostasis, including mitochondrial DNA (mtDNA) distribution. Cardiac myocytes have a specialized cytoplasmic structure where large mitochondria are aligned into tightly packed myofibril bundles; however, recent studies have revealed that mitochondrial dynamics also plays an important role in the formation and maintenance of cardiomyocytes. Here, we precisely analyzed the role of mitochondrial fission in vivo. The mitochondrial fission GTPase, Drp1, is highly expressed in the developing neonatal heart, and muscle-specific Drp1 knockout (Drp1-KO) mice showed neonatal lethality due to dilated cardiomyopathy. The Drp1 ablation in heart and primary cultured cardiomyocytes resulted in severe mtDNA nucleoid clustering and led to mosaic deficiency of mitochondrial respiration. The functional and structural alteration of mitochondria also led to immature myofibril assembly and defective cardiomyocyte hypertrophy. Thus, the dynamics of mtDNA nucleoids regulated by mitochondrial fission is required for neonatal cardiomyocyte development by promoting homogeneous distribution of active mitochondria throughout the cardiomyocytes.

Original languageEnglish
Pages (from-to)211-223
Number of pages13
JournalMolecular and cellular biology
Volume35
Issue number1
DOIs
Publication statusPublished - 2015

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

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    Ishihara, T., Ban-Ishihara, R., Maeda, M., Matsunaga, Y., Ichimura, A., Kyogoku, S., Aoki, H., Katada, S., Nakada, K., Nomura, M., Mizushima, N., Mihara, K., & Ishihara, N. (2015). Dynamics of mitochondrial DNA nucleoids regulated by mitochondrial fission is essential for maintenance of homogeneously active mitochondria during neonatal heart development. Molecular and cellular biology, 35(1), 211-223. https://doi.org/10.1128/MCB.01054-14