TY - JOUR
T1 - Dynamics of polycomb proteins-mediated histone modifications during UV irradiation-induced DNA damage
AU - Li, Zhiqing
AU - Mon, Hiroaki
AU - Mitsunobu, Hitoshi
AU - Zhu, Li
AU - Xu, Jian
AU - Lee, Jae Man
AU - Kusakabe, Takahiro
N1 - Funding Information:
We would like to thank two anonymous reviewers for their constructive comments that have improved our manuscript. This work was supported by JSPS KAKENHI Grant Number 24-02396 to ZL. The authors have declared that no conflict of interest exists.
Publisher Copyright:
© 2014 Elsevier Ltd.
PY - 2014/12/1
Y1 - 2014/12/1
N2 - Polycomb group (PcG) complexes are known to be chromatin modifiers and transcriptional repressors. In this work, we reported that the histone-modifying PcG complexes are able to participate in the repair process of ultraviolet (UV)-induced DNA lesions in the silkworm, Bombyx mori. The silkworm cells with depletion of PcG genes showed hypersensitive to UV-C irradiation and increased inhibition of cell proliferation. Interestingly, an SQ site in the silkworm-human chimeric H2A protein synthesized here was phosphorylated rapidly upon UV-C exposure, which could be used as a marker for monitoring the response to DNA damage in silkworm cells. Under these UV-C irradiated conditions, we found that PRC1-mediated ubiquitylation of H2AX, but not of H2AZ, were decreased and this deubiquitylation was independent of its phosphorylation event. In contrast, UV-C irradiation induced the increase of trimethylation of lysine 27 on histone H3 (H3K27me3), a mark of transcriptionally silent chromatin catalyzed by another PcG subcomplex, PRC2. Collectively, we provided the first evidence on chromatin remodeling in response to UV-C lesion in silkworm and revealed another layer role for PcG complexes-mediated histone modifications in contributing to creating an open chromatin structure for the efficient repair of DNA damages.
AB - Polycomb group (PcG) complexes are known to be chromatin modifiers and transcriptional repressors. In this work, we reported that the histone-modifying PcG complexes are able to participate in the repair process of ultraviolet (UV)-induced DNA lesions in the silkworm, Bombyx mori. The silkworm cells with depletion of PcG genes showed hypersensitive to UV-C irradiation and increased inhibition of cell proliferation. Interestingly, an SQ site in the silkworm-human chimeric H2A protein synthesized here was phosphorylated rapidly upon UV-C exposure, which could be used as a marker for monitoring the response to DNA damage in silkworm cells. Under these UV-C irradiated conditions, we found that PRC1-mediated ubiquitylation of H2AX, but not of H2AZ, were decreased and this deubiquitylation was independent of its phosphorylation event. In contrast, UV-C irradiation induced the increase of trimethylation of lysine 27 on histone H3 (H3K27me3), a mark of transcriptionally silent chromatin catalyzed by another PcG subcomplex, PRC2. Collectively, we provided the first evidence on chromatin remodeling in response to UV-C lesion in silkworm and revealed another layer role for PcG complexes-mediated histone modifications in contributing to creating an open chromatin structure for the efficient repair of DNA damages.
UR - http://www.scopus.com/inward/record.url?scp=84908397548&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84908397548&partnerID=8YFLogxK
U2 - 10.1016/j.ibmb.2014.10.001
DO - 10.1016/j.ibmb.2014.10.001
M3 - Article
C2 - 25308962
AN - SCOPUS:84908397548
SN - 0965-1748
VL - 55
SP - 9
EP - 18
JO - Insect Biochemistry and Molecular Biology
JF - Insect Biochemistry and Molecular Biology
ER -