Dynamics of the nucleoside diphosphate kinase protein DYNAMO2 correlates with the changes in the global GTP level during the cell cycle of Cyanidioschyzon merolae

Yuuta Imoto, Yuichi Abe, Kanji Okumoto, Mio Ohnuma, Haruko Kuroiwa, Tsuneyoshi Kuroiwa, Yukio Fujiki

Research output: Contribution to journalArticle

Abstract

GTP is an essential source of energy that supports a large array of cellular mechanochemical structures ranging from protein synthesis machinery to cytoskeletal apparatus for maintaining the cell cycle. However, GTP regulation during the cell cycle has been difficult to investigate because of heterogenous levels of GTP in asynchronous cell cycles and genetic redundancy of the GTP-generating enzymes. Here, in the unicellular red algae Cyanidioschyzon merolae, we demonstrated that the ATP-GTP-converting enzyme DYNAMO2 is an essential regulator of global GTP levels during the cell cycle. The cell cycle of C. merolae can be highly synchronized by light/dark stimulations to examine GTP levels at desired time points. Importantly, the genome of C. merolae encodes only two isoforms of the ATP-GTP-converting enzyme, namely DYNAMO1 and DYNAMO2. DYNAMO1 regulates organelle divisions, whereas DYNAMO2 is entirely localized in the cytoplasm. DYNAMO2 protein levels increase during the S-M phases, and changes in GTP levels are correlated with these DYNAMO2 protein levels. These results indicate that DYNAMO2 is a potential regulator of global GTP levels during the cell cycle.

Original languageEnglish
Pages (from-to)75-85
Number of pages11
JournalProceedings of the Japan Academy Series B: Physical and Biological Sciences
Volume95
Issue number2
DOIs
Publication statusPublished - Jan 1 2019

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Nucleoside-Diphosphate Kinase
nucleoside-diphosphate kinase
diphosphates
nucleosides
Guanosine Triphosphate
global change
cell cycle
Cell Cycle
proteins
cycles
Proteins
enzymes
adenosine triphosphate
regulators
protein synthesis
cell structures
organelles
cytoplasm
genome
Enzymes

All Science Journal Classification (ASJC) codes

  • Agricultural and Biological Sciences(all)
  • Physics and Astronomy(all)

Cite this

Dynamics of the nucleoside diphosphate kinase protein DYNAMO2 correlates with the changes in the global GTP level during the cell cycle of Cyanidioschyzon merolae. / Imoto, Yuuta; Abe, Yuichi; Okumoto, Kanji; Ohnuma, Mio; Kuroiwa, Haruko; Kuroiwa, Tsuneyoshi; Fujiki, Yukio.

In: Proceedings of the Japan Academy Series B: Physical and Biological Sciences, Vol. 95, No. 2, 01.01.2019, p. 75-85.

Research output: Contribution to journalArticle

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