E-cadherin gene-engineered feeder systems for supporting undifferentiated growth of mouse embryonic stem cells

Masanobu Horie, Akira Ito, Takehiko Kiyohara, Yoshinori Kawabe, Masamichi Kamihira

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)

Abstract

Conventionally, embryonic stem (ES) cells are cultured on a cell layer of mouse embryonic fibroblasts (MEFs) as feeder cells to support undifferentiated growth of ES cells. In this study, cell-cell interactions between mouse ES and feeder cells were artificially engineered via an epithelial cell adhesion molecule, E-cadherin, whose expression is considerable in ES cells. Mouse mesenchymal STO and NIH3T3 cells that were genetically engineered to express E-cadherin were used in ES cell cultures as feeder cells. ES cells cultured on the E-cadherin-expressing feeder cells maintained the expression of stem cell markers, alkaline phosphatase (AP), Oct3/4, Nanog and Sox2, and the efficiency of AP-positive colony formation was comparable to MEFs, and much better than parental STO and NIH3T3 cells. Furthermore, ES cells maintained on the E-cadherin-expressing feeder cells possessed the ability to differentiate into the three germ layers both in vitro and in vivo. The results indicated that E-cadherin expression in feeder cells could improve the performance of feeder cells, which may be further applicable to create new artificial feeder cell lines.

Original languageEnglish
Pages (from-to)582-587
Number of pages6
JournalJournal of Bioscience and Bioengineering
Volume110
Issue number5
DOIs
Publication statusPublished - Nov 2010

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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