E-cadherin nuclear staining is useful for the diagnosis of ovarian adult granulosa cell tumor

Yoshihiro Ohishi, Shuichi Kurihara, Tadahisa Takeuchi, murasaki aman, Tsunehisa Kaku, Hiroaki Kobayashi, Norio Wake, Yoshinao Oda

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

We recently have demonstrated nuclear localization of E-cadherin in ovarian adult granulosa cell tumors (Histopathology 2011;58:423). The purpose of the present study is to investigate the diagnostic utility of E-cadherin nuclear staining for the differential diagnosis between ovarian adult granulosa cell tumor and its morphological mimics. Tissue samples taken from 81 ovarian tumors and 20 extraovarian tumors were immunohistochemically stained using monoclonal anti-E-cadherin antibody recognizing cytoplasmic domain (clone 36 supplied by BD Biosciences, San Jose, CA). The ovarian tumors consisted of 30 adult granulosa cell tumors, 3 Sertoli-stromal cell tumors, 14 fibrothecomas, 5 carcinoid tumors, 1 large cell neuroendocrine carcinoma, 18 endometrioid adenocarcinomas, and 10 poorly differentiated serous adenocarcinomas. Extraovarian tumors consisted of 16 uterine endometrial stromal neoplasms and 4 pulmonary small cell carcinomas. Only tumor cells with nuclear staining were considered positive in this study. Ninety percent of adult granulosa cell tumors, 67% of Sertoli-stromal cell tumors, 64% of fibrothecomas, 75% of endometrial stromal neoplasms, 75% of small cell carcinomas, and the one large cell neuroendocrine carcinoma showed E-cadherin nuclear expression, whereas all the ovarian carcinoid tumors, endometrioid adenocarcinomas, and poorly differentiated serous adenocarcinomas were negative. E-cadherin nuclear staining is useful in distinguishing between adult granulosa cell tumors and ovarian adenocarcinomas or carcinoid tumors. However, it is of limited use for distinguishing between adult granulosa cell tumors and endometrial stromal neoplasms or small cell carcinomas. E-cadherin should be included in the immunohistochemical panel for an accurate diagnosis of ovarian adult granulosa cell tumors.

Original languageEnglish
Pages (from-to)808-817
Number of pages10
JournalHuman Pathology
Volume43
Issue number6
DOIs
Publication statusPublished - Jun 1 2012

Fingerprint

Granulosa Cell Tumor
Cadherins
Staining and Labeling
Small Cell Carcinoma
Carcinoid Tumor
Endometrial Neoplasms
Sertoli Cell Tumor
Endometrioid Carcinoma
Neuroendocrine Carcinoma
Large Cell Carcinoma
Adenocarcinoma
Neoplasms
Stromal Cells
Uterine Neoplasms
Differential Diagnosis
Clone Cells
Lung
Antibodies

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine

Cite this

E-cadherin nuclear staining is useful for the diagnosis of ovarian adult granulosa cell tumor. / Ohishi, Yoshihiro; Kurihara, Shuichi; Takeuchi, Tadahisa; aman, murasaki; Kaku, Tsunehisa; Kobayashi, Hiroaki; Wake, Norio; Oda, Yoshinao.

In: Human Pathology, Vol. 43, No. 6, 01.06.2012, p. 808-817.

Research output: Contribution to journalArticle

Ohishi, Y, Kurihara, S, Takeuchi, T, aman, M, Kaku, T, Kobayashi, H, Wake, N & Oda, Y 2012, 'E-cadherin nuclear staining is useful for the diagnosis of ovarian adult granulosa cell tumor', Human Pathology, vol. 43, no. 6, pp. 808-817. https://doi.org/10.1016/j.humpath.2011.07.018
Ohishi, Yoshihiro ; Kurihara, Shuichi ; Takeuchi, Tadahisa ; aman, murasaki ; Kaku, Tsunehisa ; Kobayashi, Hiroaki ; Wake, Norio ; Oda, Yoshinao. / E-cadherin nuclear staining is useful for the diagnosis of ovarian adult granulosa cell tumor. In: Human Pathology. 2012 ; Vol. 43, No. 6. pp. 808-817.
@article{f88208a08a274facbfd9e852dce27de9,
title = "E-cadherin nuclear staining is useful for the diagnosis of ovarian adult granulosa cell tumor",
abstract = "We recently have demonstrated nuclear localization of E-cadherin in ovarian adult granulosa cell tumors (Histopathology 2011;58:423). The purpose of the present study is to investigate the diagnostic utility of E-cadherin nuclear staining for the differential diagnosis between ovarian adult granulosa cell tumor and its morphological mimics. Tissue samples taken from 81 ovarian tumors and 20 extraovarian tumors were immunohistochemically stained using monoclonal anti-E-cadherin antibody recognizing cytoplasmic domain (clone 36 supplied by BD Biosciences, San Jose, CA). The ovarian tumors consisted of 30 adult granulosa cell tumors, 3 Sertoli-stromal cell tumors, 14 fibrothecomas, 5 carcinoid tumors, 1 large cell neuroendocrine carcinoma, 18 endometrioid adenocarcinomas, and 10 poorly differentiated serous adenocarcinomas. Extraovarian tumors consisted of 16 uterine endometrial stromal neoplasms and 4 pulmonary small cell carcinomas. Only tumor cells with nuclear staining were considered positive in this study. Ninety percent of adult granulosa cell tumors, 67{\%} of Sertoli-stromal cell tumors, 64{\%} of fibrothecomas, 75{\%} of endometrial stromal neoplasms, 75{\%} of small cell carcinomas, and the one large cell neuroendocrine carcinoma showed E-cadherin nuclear expression, whereas all the ovarian carcinoid tumors, endometrioid adenocarcinomas, and poorly differentiated serous adenocarcinomas were negative. E-cadherin nuclear staining is useful in distinguishing between adult granulosa cell tumors and ovarian adenocarcinomas or carcinoid tumors. However, it is of limited use for distinguishing between adult granulosa cell tumors and endometrial stromal neoplasms or small cell carcinomas. E-cadherin should be included in the immunohistochemical panel for an accurate diagnosis of ovarian adult granulosa cell tumors.",
author = "Yoshihiro Ohishi and Shuichi Kurihara and Tadahisa Takeuchi and murasaki aman and Tsunehisa Kaku and Hiroaki Kobayashi and Norio Wake and Yoshinao Oda",
year = "2012",
month = "6",
day = "1",
doi = "10.1016/j.humpath.2011.07.018",
language = "English",
volume = "43",
pages = "808--817",
journal = "Human Pathology",
issn = "0046-8177",
publisher = "W.B. Saunders Ltd",
number = "6",

}

TY - JOUR

T1 - E-cadherin nuclear staining is useful for the diagnosis of ovarian adult granulosa cell tumor

AU - Ohishi, Yoshihiro

AU - Kurihara, Shuichi

AU - Takeuchi, Tadahisa

AU - aman, murasaki

AU - Kaku, Tsunehisa

AU - Kobayashi, Hiroaki

AU - Wake, Norio

AU - Oda, Yoshinao

PY - 2012/6/1

Y1 - 2012/6/1

N2 - We recently have demonstrated nuclear localization of E-cadherin in ovarian adult granulosa cell tumors (Histopathology 2011;58:423). The purpose of the present study is to investigate the diagnostic utility of E-cadherin nuclear staining for the differential diagnosis between ovarian adult granulosa cell tumor and its morphological mimics. Tissue samples taken from 81 ovarian tumors and 20 extraovarian tumors were immunohistochemically stained using monoclonal anti-E-cadherin antibody recognizing cytoplasmic domain (clone 36 supplied by BD Biosciences, San Jose, CA). The ovarian tumors consisted of 30 adult granulosa cell tumors, 3 Sertoli-stromal cell tumors, 14 fibrothecomas, 5 carcinoid tumors, 1 large cell neuroendocrine carcinoma, 18 endometrioid adenocarcinomas, and 10 poorly differentiated serous adenocarcinomas. Extraovarian tumors consisted of 16 uterine endometrial stromal neoplasms and 4 pulmonary small cell carcinomas. Only tumor cells with nuclear staining were considered positive in this study. Ninety percent of adult granulosa cell tumors, 67% of Sertoli-stromal cell tumors, 64% of fibrothecomas, 75% of endometrial stromal neoplasms, 75% of small cell carcinomas, and the one large cell neuroendocrine carcinoma showed E-cadherin nuclear expression, whereas all the ovarian carcinoid tumors, endometrioid adenocarcinomas, and poorly differentiated serous adenocarcinomas were negative. E-cadherin nuclear staining is useful in distinguishing between adult granulosa cell tumors and ovarian adenocarcinomas or carcinoid tumors. However, it is of limited use for distinguishing between adult granulosa cell tumors and endometrial stromal neoplasms or small cell carcinomas. E-cadherin should be included in the immunohistochemical panel for an accurate diagnosis of ovarian adult granulosa cell tumors.

AB - We recently have demonstrated nuclear localization of E-cadherin in ovarian adult granulosa cell tumors (Histopathology 2011;58:423). The purpose of the present study is to investigate the diagnostic utility of E-cadherin nuclear staining for the differential diagnosis between ovarian adult granulosa cell tumor and its morphological mimics. Tissue samples taken from 81 ovarian tumors and 20 extraovarian tumors were immunohistochemically stained using monoclonal anti-E-cadherin antibody recognizing cytoplasmic domain (clone 36 supplied by BD Biosciences, San Jose, CA). The ovarian tumors consisted of 30 adult granulosa cell tumors, 3 Sertoli-stromal cell tumors, 14 fibrothecomas, 5 carcinoid tumors, 1 large cell neuroendocrine carcinoma, 18 endometrioid adenocarcinomas, and 10 poorly differentiated serous adenocarcinomas. Extraovarian tumors consisted of 16 uterine endometrial stromal neoplasms and 4 pulmonary small cell carcinomas. Only tumor cells with nuclear staining were considered positive in this study. Ninety percent of adult granulosa cell tumors, 67% of Sertoli-stromal cell tumors, 64% of fibrothecomas, 75% of endometrial stromal neoplasms, 75% of small cell carcinomas, and the one large cell neuroendocrine carcinoma showed E-cadherin nuclear expression, whereas all the ovarian carcinoid tumors, endometrioid adenocarcinomas, and poorly differentiated serous adenocarcinomas were negative. E-cadherin nuclear staining is useful in distinguishing between adult granulosa cell tumors and ovarian adenocarcinomas or carcinoid tumors. However, it is of limited use for distinguishing between adult granulosa cell tumors and endometrial stromal neoplasms or small cell carcinomas. E-cadherin should be included in the immunohistochemical panel for an accurate diagnosis of ovarian adult granulosa cell tumors.

UR - http://www.scopus.com/inward/record.url?scp=84861186672&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84861186672&partnerID=8YFLogxK

U2 - 10.1016/j.humpath.2011.07.018

DO - 10.1016/j.humpath.2011.07.018

M3 - Article

C2 - 22079356

AN - SCOPUS:84861186672

VL - 43

SP - 808

EP - 817

JO - Human Pathology

JF - Human Pathology

SN - 0046-8177

IS - 6

ER -