E1A and ras Oncogenes Synergistically Enhance Recombinant Protein Production under Control of the Cytomegalovirus Promoter in BHK-21 Cells

Sanetaka Shirahata, Junko Watanabe, Kiichiro Teruya, Takahiro Yano, Kazuhiro Osada, Hirofumi Tachibana, Hiroki Murakami, Hideya Ohashi, Eun Ho Kim

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)

Abstract

The amplified ras oncogene greatly enhanced the production of recombinant human interleukin-6 (hIL-6) under control of the cytomegalovirus immediate early promoter (CMV promoter) in BHK-21 cells. When the adenovirus E1A oncogene was further transfected into the above mentioned ras-amplified hIL-6 hyper-producing BHK cells, the transfectants had about 10 times higher productivity than non-transfectants. However, the E1A gene alone did not enhance productivity. These results implicate a ras and E1A synergistic ability that acts to enhance hIL-6 production.

Original languageEnglish
Pages (from-to)345-347
Number of pages3
JournalBioscience, biotechnology, and biochemistry
Volume59
Issue number2
DOIs
Publication statusPublished - 1995

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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