E1A and ras Oncogenes Synergistically Enhance Recombinant Protein Production under Control of the Cytomegalovirus Promoter in BHK-21 Cells

Sanetaka Shirahata; Junko Watanabe; Kiichiro Teruya; Takahiro Yano; Kazuhiro Osada; Hirofumi Tachibana; Hiroki Murakami; Hideya Ohashi; Eun Ho Kim

doi:10.1271/bbb.59.345

E1A and ras Oncogenes Synergistically Enhance Recombinant Protein Production under Control of the Cytomegalovirus Promoter in BHK-21 Cells

Sanetaka Shirahata, Junko Watanabe, Kiichiro Teruya, Takahiro Yano, Kazuhiro Osada, Hirofumi Tachibana, Hiroki Murakami, Hideya Ohashi, Eun Ho Kim

Research output: Contribution to journal › Article › peer-review

18 Citations (Scopus)

Abstract

The amplified ras oncogene greatly enhanced the production of recombinant human interleukin-6 (hIL-6) under control of the cytomegalovirus immediate early promoter (CMV promoter) in BHK-21 cells. When the adenovirus E1A oncogene was further transfected into the above mentioned ras-amplified hIL-6 hyper-producing BHK cells, the transfectants had about 10 times higher productivity than non-transfectants. However, the E1A gene alone did not enhance productivity. These results implicate a ras and E1A synergistic ability that acts to enhance hIL-6 production.

Original language	English
Pages (from-to)	345-347
Number of pages	3
Journal	Bioscience, biotechnology, and biochemistry
Volume	59
Issue number	2
DOIs	https://doi.org/10.1271/bbb.59.345
Publication status	Published - 1995

All Science Journal Classification (ASJC) codes

Biotechnology
Analytical Chemistry
Biochemistry
Applied Microbiology and Biotechnology
Molecular Biology
Organic Chemistry

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title = "E1A and ras Oncogenes Synergistically Enhance Recombinant Protein Production under Control of the Cytomegalovirus Promoter in BHK-21 Cells",

abstract = "The amplified ras oncogene greatly enhanced the production of recombinant human interleukin-6 (hIL-6) under control of the cytomegalovirus immediate early promoter (CMV promoter) in BHK-21 cells. When the adenovirus E1A oncogene was further transfected into the above mentioned ras-amplified hIL-6 hyper-producing BHK cells, the transfectants had about 10 times higher productivity than non-transfectants. However, the E1A gene alone did not enhance productivity. These results implicate a ras and E1A synergistic ability that acts to enhance hIL-6 production.",

author = "Sanetaka Shirahata and Junko Watanabe and Kiichiro Teruya and Takahiro Yano and Kazuhiro Osada and Hirofumi Tachibana and Hiroki Murakami and Hideya Ohashi and Kim, {Eun Ho}",

note = "Funding Information: Acknowledgment. We would like to thank Dr. K. Onodera of the University of Tokyo, for generously providing the pSV2-gpt-gEIA plasmid.",

year = "1995",

doi = "10.1271/bbb.59.345",

language = "English",

volume = "59",

pages = "345--347",

journal = "Bioscience, Biotechnology and Biochemistry",

issn = "0916-8451",

publisher = "Japan Society for Bioscience Biotechnology and Agrochemistry",

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TY - JOUR

T1 - E1A and ras Oncogenes Synergistically Enhance Recombinant Protein Production under Control of the Cytomegalovirus Promoter in BHK-21 Cells

AU - Shirahata, Sanetaka

AU - Watanabe, Junko

AU - Teruya, Kiichiro

AU - Yano, Takahiro

AU - Osada, Kazuhiro

AU - Tachibana, Hirofumi

AU - Murakami, Hiroki

AU - Ohashi, Hideya

AU - Kim, Eun Ho

N1 - Funding Information: Acknowledgment. We would like to thank Dr. K. Onodera of the University of Tokyo, for generously providing the pSV2-gpt-gEIA plasmid.

PY - 1995

Y1 - 1995

N2 - The amplified ras oncogene greatly enhanced the production of recombinant human interleukin-6 (hIL-6) under control of the cytomegalovirus immediate early promoter (CMV promoter) in BHK-21 cells. When the adenovirus E1A oncogene was further transfected into the above mentioned ras-amplified hIL-6 hyper-producing BHK cells, the transfectants had about 10 times higher productivity than non-transfectants. However, the E1A gene alone did not enhance productivity. These results implicate a ras and E1A synergistic ability that acts to enhance hIL-6 production.

AB - The amplified ras oncogene greatly enhanced the production of recombinant human interleukin-6 (hIL-6) under control of the cytomegalovirus immediate early promoter (CMV promoter) in BHK-21 cells. When the adenovirus E1A oncogene was further transfected into the above mentioned ras-amplified hIL-6 hyper-producing BHK cells, the transfectants had about 10 times higher productivity than non-transfectants. However, the E1A gene alone did not enhance productivity. These results implicate a ras and E1A synergistic ability that acts to enhance hIL-6 production.

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SN - 0916-8451

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JO - Bioscience, Biotechnology and Biochemistry

JF - Bioscience, Biotechnology and Biochemistry

IS - 2

ER -

E1A and ras Oncogenes Synergistically Enhance Recombinant Protein Production under Control of the Cytomegalovirus Promoter in BHK-21 Cells

Abstract

All Science Journal Classification (ASJC) codes

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