Early embryogenesis in zebrafish is affected by bisphenol A exposure

William K.F. Tse, Bonnie H.Y. Yeung, H. T. Wan, Chris K.C. Wong

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Exposure of a developing embryo or fetus to endocrine disrupting chemicals (EDCs) has been hypothesized to increase the propensity of an individual to develop a disease or dysfunction in his/her later life. Although it is important to understand the effects of EDCs on early development in animals, sufficient information about these effects is not available thus far. This is probably because of the technical difficulties in tracing the continuous developmental changes at different stages of mammalian embryos. The zebrafish, an excellent model currently used in developmental biology, provides new insights to the field of toxicological studies. We used the standard whole-mount in situ hybridization screening protocol to determine the early developmental defects in zebrafish embryos exposed to the ubiquitous pollutant, bisphenol A (BPA). Three stages (60-75% epiboly, 8-10 somite, and prim-5) were selected for in situ screening of different molecular markers, whereas BPA exposure altered early dorsoventral (DV) patterning, segmentation, and brain development in zebrafish embryos within 24 hours of exposure.

Original languageEnglish
Pages (from-to)466-471
Number of pages6
JournalBiology Open
Volume2
Issue number5
DOIs
Publication statusPublished - May 15 2013

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Endocrine Disruptors
bisphenol A
Zebrafish
Danio rerio
Embryonic Development
Screening
embryo (animal)
embryogenesis
Embryonic Structures
endocrine-disrupting chemicals
Mammalian Embryo
Developmental Biology
Brain
Somites
Animals
screening
Toxicology
Defects
In Situ Hybridization
Fetus

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Early embryogenesis in zebrafish is affected by bisphenol A exposure. / Tse, William K.F.; Yeung, Bonnie H.Y.; Wan, H. T.; Wong, Chris K.C.

In: Biology Open, Vol. 2, No. 5, 15.05.2013, p. 466-471.

Research output: Contribution to journalArticle

Tse, William K.F. ; Yeung, Bonnie H.Y. ; Wan, H. T. ; Wong, Chris K.C. / Early embryogenesis in zebrafish is affected by bisphenol A exposure. In: Biology Open. 2013 ; Vol. 2, No. 5. pp. 466-471.
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