TY - JOUR
T1 - Eastern blotting and use of anti-saikosaponin a monoclonal antibodies for detection of saikosaponins
AU - Zhu, Shu Hang
AU - Morinaga, Osamu
AU - Shimokawa, Shinichi
AU - Shon, Tae Kwon
AU - Lee, Sang Chul
AU - Shoyama, Yukihiro
AU - Tanaka, Hiroyuki
N1 - Funding Information:
Acknowledgments This work was supported in part by a Grant-in-Aid from JSPS’s Asian CORE Program, the Ministry of Education, Culture, Sports, Science and Technology of Japan, the research funds of the Kyushu University Foundation, and the Japan Kampo Medicine Manufacturers Association from Japan Kampo Medicine Manufacturers.
PY - 2007/4
Y1 - 2007/4
N2 - Immunochemical assays using anti-saikosaponin a monoclonal antibody (MAb) and so-called Eastern blotting have been established for semi-quantitative analysis of the saikosaponins (SS) SSa, SSb2, SSc, and SSd, which are active constituents of the Bupleuri radix. After crude separation of saikosaponins by TLC, compounds were transferred from the TLC plate to a PVDF membrane where they were oxidized with NaIO4 and, finally, conjugated with bovine serum albumin (BSA). Clear and specific detection and identification of the immobilized saikosaponin-BSA conjugates were achieved by use of the MAbs. Compared with TLC, the Eastern blotting technique for analysis of saikosaponin compounds is a simple method for quality-control monitoring of Bupleuri radix and of Kampo formulations containing Bupleuri radix.
AB - Immunochemical assays using anti-saikosaponin a monoclonal antibody (MAb) and so-called Eastern blotting have been established for semi-quantitative analysis of the saikosaponins (SS) SSa, SSb2, SSc, and SSd, which are active constituents of the Bupleuri radix. After crude separation of saikosaponins by TLC, compounds were transferred from the TLC plate to a PVDF membrane where they were oxidized with NaIO4 and, finally, conjugated with bovine serum albumin (BSA). Clear and specific detection and identification of the immobilized saikosaponin-BSA conjugates were achieved by use of the MAbs. Compared with TLC, the Eastern blotting technique for analysis of saikosaponin compounds is a simple method for quality-control monitoring of Bupleuri radix and of Kampo formulations containing Bupleuri radix.
UR - http://www.scopus.com/inward/record.url?scp=42649121729&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=42649121729&partnerID=8YFLogxK
U2 - 10.1007/s11418-006-0104-9
DO - 10.1007/s11418-006-0104-9
M3 - Article
AN - SCOPUS:42649121729
SN - 1340-3443
VL - 61
SP - 178
EP - 183
JO - Journal of Natural Medicines
JF - Journal of Natural Medicines
IS - 2
ER -